曲霉NS-1产木聚糖酶发酵条件优化

Condition Optimization of Xylanase from Aspergillus NS-1

以真菌NS-1为研究对象,采用单因素试验研究碳源、碳源组成、氮源、碳酸钙、表面活性剂及初始p H对产木聚糖酶的影响。结果表明:最佳碳源为玉米芯;复合碳源玉米芯+木聚糖最有利于木聚糖酶的分泌;最佳氮源是硫酸铵;当初始p H为5.0,碳酸钙浓度为1.5%,表面活性剂为SDS时,木聚糖酶活力最高。

The xylanase expression of Aspergillus NS-1 was studied with the single factor experiment of carbon source,carbon source concentration,nitrogen source,calcium carbonate,surfactants and pH value. The results of the experiments showed： the optimal carbon source and nitrogen source is corn cob,ammonium sulfate,respectively. The compound carbon sources are comcob and xylan. The concentration of Calcium carbonate of 1. 5% and SDS added were optimal for expression of xylanase at pH5. 0.