小鼠孤雌胚胎及孤雌胚胎干细胞中印记基因的表达

Expression of imprinting gene in mouse parthenogenetic embryos and parthenogenetic embryonic stem cells

小鼠孤雌胚胎体内发育最多不能超过10.5d,发育失败的主要原因是胚外组织发育缺陷和印记基因表达的异常。随着小鼠孤雌二倍体和单倍体胚胎干细胞的建系成功,不仅能作为研究印记基因的理想模型,还能够作为种子细胞应用于细胞治疗,拓宽了小鼠孤雌生殖的研究领域和再生医学应用范围。孤雌胚胎聚合作为一种简便易行的技术手段,能够显著提高孤雌胚胎干细胞的建系效率,还能促使异质胚胎间的印记基因相互补偿从而更加趋近于正常受精的胚胎干细胞。我们在文中主要阐释了小鼠孤雌胚胎、孤雌聚合胚胎、孤雌二倍体胚胎干细胞、孤雌单倍体胚胎、孤雌聚合胚胎干细胞中印记基因的表达。

The development of mice parthenogenetic embryos can not be beyond 10.5days, because of the defect in extra-embryonic tissues and the abnormal expression of imprinting gene. Establishment of mice diploid or haploid parthenogenetic embryonic stem cells may be applied for searching novel imprinting genes and for cell therapy, which broaden the field of scientific research and regenerative medicine. Aggregation of parthenogenetic embryos as a convenient method not only can notably increase the efficiency of establishing parthenogenetic stem cells but also improve the expression level of imprinting genes. This improvement probably achieves through compensation of imprinting genes expression among different parthenogenetic embryos. In this paper we discuss the expression of imprinting genes in mice parthenogenetic embryos, aggregated parthenogenetic embryos, parthenogenetic haploid embryonic stem ceils, parthenogenetic diploid embryonic stem ceils and parthenogenetic embryonic stem ceils derived from aggregated embryos.