转Bar基因稻谷对小鼠(Mus musculus)肠道菌群的作用研究

Research on the Effects of Bar-transgenic Rice on the Microflora in Intestine of Mice(Mus musculus)

选取6周龄、雌雄各半、体重18～22 g的SPF级昆明小鼠（Mus musculus）100只袁随机分成5组袁每组4个重复袁每个重复5只小鼠。分别用含量为40%和60%的转Bar基因稻谷Bar68-1和非转基因稻谷D68日粮喂养小鼠袁亲代小鼠饲养180 d后开始繁殖子一代（F1）袁每代小鼠饲养180 d。在180 d后分别从亲代和子一代每组随机抽取5只小鼠袁提取肠道内容物基因组DNA袁利用细菌通用引物对细菌16S r DNA的V3区序列进行PCR扩增袁并将扩增产物用变性梯度凝胶电泳（denaturing gradient gel electrophoresis DGGE）。研究显示袁各组小鼠肠道菌群相似性系数无显著差异（P〉0.05）曰肠道主要优势菌群相同。结果表明袁转Bar基因稻谷对小鼠肠道菌群的作用不明显。

100 SPF Kunming mice （Mus musculus） （20＋2 g）, half male and half female, were randomly divided into 5 groups, with 4 replications per group and 5 mice per replication. They were fed with diets that contained low and high dose of genetically modified （GM） rice Bar68-1,non-GM rice D68 or routine feed respectively for duration of 180 days. After 90 days, parental generation （P） bred the first filial generation （F1）. Each generation was fed for 180 days respectively. On the 180th day, 5 mice of each group were sampled at random, and intestinal contents were collected for DNA isolation. The V3 region of 16S rDNA was amplified by PCR and analyzed via denaturing gradient gel electrophoresis （DGGE）. Cs values for intergroup comparisons of samples did not show significant difference from each other （P〉0.05）, and some identical intestinal predominant flora was identified by sequence analysis of bands. The results showed that GM rice Bar68-1 had no significant effects on the microflora in Mus musculus intestine.