响应面法优化硫酸盐还原菌培养条件

Optimization of Culture Conditions for Sulfate-Reducing Bacteria by Response Surface Methodology

采用Box-Behnken中心组合设计及响应面法(Response surface methodology)对影响硫酸盐还原茵(SRB)生长的半胱氨酸含量、初始pH值和Na_2SO_4含量3个主要因子的最佳水平及其交互作用进行了研究与探讨,并经Design-Expert.8.05B软件分析数据。结果表明:影响SRB菌株反硫化率的主次因素依次为:半胱氨酸浓度、初始pH值、硫酸钠浓度;三因素中两两因素的交互作用均不显著;最佳培养条件为半胱氨酸质量分数0.01%、初始pH值8.0、硫酸钠质量浓度2.0g/L。在此条件下,SRB菌株的反硫化率平均值为10.642%,与预测值10.097%接近,说明所建立的模型是切实可行的。

Box-Behnken and response surface methodology were used to research and probe the best level and mutual effect of cysteine concentration,pH value and Na2SO4 concentration that affected the growth of sulfate-reducing bacteria（SRB）.The data was analyzed by Design-Expert.8.05 B software.The results showed that three factors were arranged according to their influence on anti-vulcanization rate of SRB in following order：cysteine concentration,medium pH and sodium sulfate concentration.And the interaction between two factors in fitted regression model had insignificant effect on anti-vulcanization rate of SRB.The optimum culture condition was obtained as follows：0.01%cysteine mass fraction,8.0 pH value and 2.0 g/L sodium sulfate concentration.Under this condition,the average anti-vulcanization rate of SRB was 10.642%,which was close to the predicted value（10.097%）.Therefore,it was feasible for the established model due to the consistent result between prediction and experiment.