加味丹参饮对动脉粥样硬化模型小鼠内皮细胞功能及超微结构的影响

Effects of Jiawei Danshen Decoction on Endothelial Cell Function and Ultrastructure in Atherosclerosis Model Mice

目的 观察加味丹参饮对ApoE-/-动脉粥样硬化（AS）模型小鼠血清一氧化氮（NO）和内皮素（ET）及内皮细胞超微结构的影响,探讨其抗AS 作用机制.方法 24 只7～8 周龄ApoE-/-小鼠饲以高脂饲料12 周造模,随机分为模型组、阳性药组和加味丹参饮低、高剂量组,每组6 只;同品系C57BL/6J 小鼠作为空白组,饲以普通饲料.药物干预8 周后,摘眼球取血测NO、ET 水平,透射电镜观察主动脉内皮细胞超微结构变化.结果 与空白组比较,模型组血清NO 含量明显降低、ET 含量明显升高（P 〈0.01）;与模型组比较,各给药组NO 含量明显升高、ET 含量明显降低（P 〈0.05,P 〈0.01）.电镜结果显示,模型组呈AS 脂纹期表现,各给药组内皮损伤较模型组均有改善.结论 加味丹参饮可改善内皮细胞超微结构、功能,保护内皮细胞,延缓AS 进程.

Objective To investigate the effects of Jiawei Danshen Decoction on endothelial cell ultrastructure, serum NO and ET in apolipoprotein E-knockout atherosclerosis(AS) mice;To discuss its mechanism of anti-atherosclerosis. Methods Totally 24 7-8 weeks old Apo E-/-mice were fed with a high fat diet for 12 weeks until the a mature atherosclerotic plaque was formed. They were randomly divided into four groups:model group, control group, Jiawei Danshen Decoction of low-dose group and high-dose group, 6 mice in each group. Another 6 normal C57BL/6J mice with the same strain were set as the blank group, and fed with general diet. After medicine intervention by gavage for 8 weeks, blood was extracted from orbital venous to measure serum NO and ET;aortic endothelial cell ultrastructure changes were observed by transmission electron microscope. Results Compared with the blank group, the level of NO obviously decreased and ET significantly increased in the model group(P < 0.01). Compared with model group, medicine intervention increased NO and reduce ET level(P <0.05). Electron microscopy results showed that the model group showed fatty streaks stage performance;endothelial damage in each administration group was improved compared with model group. Conclusion Jiawei Danshen Decoction can improve the endothelial cell ultrastructure and functions and protect endothelial cells, with a purpose to delay the AS process.