8周间歇运动和G-CSF动员对心梗大鼠心肌组织VEGF/VEGFR-2的影响

Effects of Eight Weeks Interval Exercise and G-CSF Mobilization on VEGF/VEGFR-2 of Myocardial Tissue in Rats with Myocardial Infarction

目的：探讨8周间歇运动和G-CSF动员对心梗（MI）大鼠心肌血管新生数量及血管再生通路因子VEGF/VEGFR-2的影响。方法：3月龄雄性SD大鼠,体质量185~210 g,结扎LAD建立MI模型。术后存活的大鼠随机分为假手术对照组（A组）、MI组（B组）、间歇运动＋MI组（C组）、动员剂＋MI组（D组）和间歇运动＋动员剂＋MI组（E组）,每组10只。C组和E组进行8周跑台间歇运动训练,8周后免疫组织化学方法染色测定VEGF、VEGFR-2表达量和CD31表达数量,用血管墨汁灌注法对MI区血管形成情况进行观察。结果：免疫组化结果显示,各干预手段均可上调MI大鼠心肌VEGF、VEGFR-2、CD31表达（数）量,且E组〉D组〉C组;血管墨汁染色结果显示,各干预手段均可促进MI区血管、血管网的形成。结论：间歇运动和/或G-CSF均显著上调了MI大鼠心肌血管再生通路因子VEGF/VEGFR的表达,促进血管再生,增加血管新生数量,且间歇运动联合G-CSF动员的双重作用效果更佳,可能与2者有效动员内皮祖细胞（EPCs）数量和能力,参与、分化为新生血管有关。该研究为有效地治疗缺血性心脏病提供基础实验依据。

Objectives： This study explored the effects of interval exercise and granulocyte colony-stimulating factor（ G-CSF） mobilization on angiogenesis density and the expression of angiogenesis pathway factor VEGF/VEGFR-2of myocardial tissue in rats with MI. Methods： Male 3-month old SD rats weighted 185- 210 g were subjects and were established MI model by ligation of the left anterior descending coronary artery（ LAD）. Survival rats were randomly divided into sham-operated group（ group A）,sedentary MI group（ group B）,interval exercise with MI group（ group C）,G-CSF with MI group（ group D） and interval exercise with G-CSF plus MI group（ group E）,10 rats in each group. Rats in group C and group E were underwent 8-week treadmill interval exercise. After exercise,the expressions of VEGF and VEGFR-2 and number of CD31 in myocardial tissue were tested by Immunohistochemistry,and the formation of blood vessels in MI area was observed by ink perfusion method. Results： The results of Immunohistochemistry indicated that all the intervention can improve the expressions of VEGF and VEGFR-2,and number of CD31 in myocardial tissue,and they were highest in E group,following by D group,and C group was the lowest one. The results of ink perfusion method showed that all the interventions can promote vascular and vascular network formation in MI area. Conclusions： Interval exercise or G-CSF can promote the expression of vascular regeneration pathway of VEGF / VEGFR-2,accelerate angiogenesis,and combining both of them has better effect,which is related to that both of them can improve the mobilization and differentiation of endothelial progenitor cells（ EPCS）. This study provides experimental basis for treatment of ischemic heart disease.