社区获得性肺炎蛋白指纹图谱诊断技术研究

Identification of diagnosis markers for community acquired pneumonia by proteomic finger-printing

目的探索社区获得性肺炎蛋白指纹图谱的特点。方法从本院临床病例中,选择社区获得性细菌性肺炎患者与健康者各60例,进行血清蛋白指纹图谱检测,分析其相关蛋白峰值并进行统计学处理。结果对60例社区获得性细菌性肺炎患者与60例健康者的血清蛋白指纹图谱数据进行比较,发现有3个蛋白峰(1028.49、4796.56、7564.77m/z)存在显著的差异(P<0.01)。由此3个蛋白峰组成的诊断模型判别社区获得性细菌性肺炎的总有效率为95.8%(115/120),特异度为92.3%(60/65)、灵敏度为100%(55/55)、阳性预测值为91.7%(55/60)、阴性预测值为100%(60/60)。结论蛋白质指纹图谱技术具有方法简便、检测快速,标本用量少等优点,可能成为社区获得性肺炎早期诊断的辅助指标。

Objective To explore the characteristics of community acquired pneumonia using proteinfingerprinting technology. Method 60 patients with community acquired bacterial pneumonia,and 60 healthy volunteers were selected from known clinical cases. Serum protein fingerprint studies were used to analyze their protein peaks and perform statistical processing. Result Comparison of the serum protein fingerprinting data from the pool of 60 patients and 60 healthy volunteers,Significant difference in 3 protein peaks（ 1028. 49、4796. 56、7564. 77 m / z） identified between community acquired bacterial pneumonia and healthy volunteers（ P〈0. 01）. The total effective rate of the 3protein peaks as a diagnosis model for differential diagnosis of community acquired bacterial pneumonia and healthy volunteers was 95. 8%（ 115 /120）,The specificity was 92. 3%（ 60 /65）,the sensitivity was 100%（ 55 /55）,The positive predictive value was 91. 7%（ 55 /60）,the negative predictive value was 100%（ 60 /60）. Conclusion Protein fingerprinting technology is advantageous of being a simple method,quick detection,and requires less amount of sample. It is expected to become the early auxiliary diagnosis index for differential diagnosis of community acquired pneumonia.