脑白质疏松与空间学习及记忆功能障碍关系的研究

Association of leukoaraiosis with spatial learning and memory dysfunction

目的探讨脑白质疏松与空间学习及记忆功能障碍的关系。方法将60只雄性SD大鼠随机分为假手术组(n=30)和模型组(n=30),通过结扎双侧颈总动脉构建脑白质疏松模型,术前及术后3月分别进行Morris水迷宫实验,同时观察脑白质的组织学变化。结果定向航行阶段,与假手术组相比,模型组寻找隐敝平台能力下降(P<0.05),且于术后第2月开始出现(P<0.05),第3月改变明显(P<0.01);空间探索阶段,2组穿越次数差异无统计学意义(P>0.05);站台周边1路程显示,模型组记忆能力较假手术组降低,此种改变于术后第3月出现(P<0.05)。模型组脑白质病理改变显示质地疏松,出现大量空泡。结论脑白质疏松出现一定时间之后可导致空间学习及记忆障碍,并且空间学习能力受损早于记忆能力下降。

Objective To investigate the association of leukoaraiosis with spatial learning and memory dysfunction.Methods Sixty male SD rats were randomly divided into sham group and model group with 30 rats in each group.Leukoaraiosis model was constructed by bilateral carotid artery ligation. The rats should undergo Morris water maze test for4 times. Histological changes of the white matter were observed. Results In directional navigation phase,compared with the sham group,learning ability of model group decreased（ P〈0. 05） which began from the second month（ P〈0. 05）,and decreased significantly in the third month（ P〈0. 01）. In space exploration phase,crossing times of the two groups were of no difference（ P〉0. 05）. The data of platform peripheral 1 indicated that memory platform was significantly enhanced in sham group（ P〈0. 01）,but weakened in model group. Memory impairment of model group appeared in the third month. Pathology in the model group showed that white matter density decreased and vacuoles appeared.Conclusions Leukoaraiosis could lead to spatial learning and memory disorders. The impairment of spatial learning appeared earlier than memory decline.