藏鸡PPARα基因克隆与生物信息学分析

Cloning and bioinformatics analysis of PPARα gene in Tibetan chicken

为阐明藏鸡PPARα基因的结构及功能,利用RT-PCR方法对PPARα基因进行克隆,并进行生物信息学分析.结果表明:克隆获得包括完整开放阅读框的藏鸡PPARα基因序列1430 bp,开放阅读框1404 bp,编码468个氨基酸;藏鸡PPARα蛋白分子量为52.23 ku,等电点为5.85,为不稳定酸性蛋白,存在27个磷酸化位点和4个糖基化位点,无信号肽和跨膜螺旋结构.预测其二级结构中α-螺旋占37.61%,β-折叠占16.67%,无规则卷曲比例占45.73%.该研究结果为揭示PPARα在藏鸡脂代谢中的作用奠定了基础.

In order to clarify the structure and reveal the role of PPARα in Tibetan chicken,the gene sequence of PPARα in Tibetan chicken was amplified by RT-PCR,and analyzed by bioinformatics methods. The results showed that the cDNA sequences of Tibetan chicken PPARα was1430 bp,containing 1404 bp of open reading frame（ ORF）,encoding 468 amino acids. The molecular weight of PPARα protein was predicted to be 52. 23 ku,and its theoretical isoelectric point was 5. 85. The protein of PPARαwas computed to be unstable and belonged to be an acidity nucleoproteins with 27 phosphorylation sites and 4 O-glycosylation sites. No a signal peptides and a transmembrane structure was observed in PPARα protein. For the secondary structure,the protein of PPARα was predicted to be composed of α-helix（ 37. 61%）,β sheet（ 16. 67%） and random coil（ 45. 73%）. These results will be beneficial for the functional research of PPARα gene in fat metabolism of Tibetan chicken.