参芎滴丸对心肌缺血大鼠血管内皮细胞及能量代谢的影响

Effect of Shenxiong Drop Pills on Vessel Endothelioid Cell and Energy Metabolism in Rats of Acute Myocardial Ischemia

目的:探讨参芎滴丸对异丙肾上腺素诱导大鼠心肌缺血的保护作用及其机制。方法:SD大鼠随机分为正常组,模型组,复方丹参滴丸组,美托洛尔组和参芎滴丸高、中、低剂量组(11.2,5.6,2.8 g·kg-1)。采用注射异丙肾上腺素(ISO)建立大鼠急性心肌缺血模型,观察参芎滴丸预给药5 d后对大鼠不同时间点心电图(ECG)的影响;继续给药2 d检测各组大鼠血清中一氧化氮合酶(NOS),一氧化氮(NO),内皮素-1(ET-1),血栓素B2(TXB2)和6-酮前列腺素(6-Keto-PGFlα)的水平;同时测定大鼠心肌组织中钙-镁-ATP(Ca2+-Mg2+-ATP)酶,钠-钾-ATP(Na+-K+-ATP)酶活性及ATP含量。结果:参芎滴丸能明显改善ISO引起的大鼠心电图J点下移变化;降低大鼠血清中ET-1,TXB2的水平;不同程度地升高血清中NOS,NO和6-KetoPGFlα的水平;但对心肌组织中ATP含量,ATP酶活性无明显影响。结论:参芎滴丸对大剂量异丙肾上腺素引起的大鼠急性缺血性心肌损伤有明显的保护作用,其机制可能与保护血管内皮细胞,抑制血小板聚集有关。

Objective： To study the protective effect and its mechanism of Shenxiong drop pills（ SDP）on the acute myocardial ischemia（ AMI） induced by isoprenaline（ ISO）. Method： Sprogue-Dawley rats were divided randomly into seven groups,with 10 in each group. They were normal group,model group,Danshen group,metoprolol group,high,medium and low-dose SDP groups（ 11. 2,5. 6,2. 8 g·kg^-1）. The AMI rat model was established by injection with ISO. Five days later,the effect of SDP on the electrocardiogram（ ECG） at different time points were observed,after the continuos administration for another two days,the activities of Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase in myocardial tissue,the levels of endothelin-1（ ET-1）,nitric oxide（ NO）,thromboxane B2（ TXB2）,6-keto-prostaglandin F1a（ 6-keto-PGF1α） and the activities of nitric oxide synthase（ NOS） in serum were detected. Meanwhile,Ca^2＋-Mg^2＋-ATP enzyme,Na^＋-K^＋-ATP enzymatic activity and ATP content in rat myocardial tissues were determined. Result： SDP inhibited the J spot downward of ECG induced by ISO during myocardial ischemia,decreased the levels of ET-1,TXB2 in serum,and increased the levels of NO,6-Keto-PGFlαand the activities of NOS in serum to varying degrees,but with no significant effect on ATP content in myocardial tissues and ATP enzymatic activity. Conclusion： The SDP has the apparent protective effect on AMI injury induced by ISO,its mechanism may be correlated with protecting endothelial cell and inhibiting platelet aggregation.