斜发沸石对亚急性辐射损伤小鼠的保护作用研究

Study on radioprotection effects of clinoptilolite on sub-acute radiation-injured mice

目的研究斜发沸石(Cp)对亚急性辐射损伤小鼠的防护作用。方法 48只雄性BALB/c小鼠随机分为空白对照组(给予蒸馏水,不照射)、辐照模型组(给予蒸馏水,照射)、300mg/kg 523组(照射前24h给予尼尔雌醇200mg/kg,照射后4h给予尼尔雌醇100mg/kg,其余天数给予蒸馏水灌胃,照射)、56mg/kg沸石组(给予56mg/kg沸石水溶液,照射)、167mg/kg沸石组(给予167mg/kg沸石水溶液,照射)和500mg/kg沸石组(给予500mg/kg沸石水溶液,照射)。以灌胃方式连续给药7d,采用137Csγ射线对小鼠进行一次性全身照射,剂量率为0.75Gy/min,总剂量为4.0Gy。照射后继续灌胃给药14d。用血细胞分析仪测定外周血象;测定脏器指数、骨髓DNA含量;检测小鼠血清超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量及谷胱甘肽过氧化物酶(GSH-Px)活性;流式细胞仪检测骨髓造血干细胞含量。结果经137Csγ射线照射后10d,与辐照模型组相比,56mg/kg沸石组与167mg/kg沸石组红细胞数量明显升高(P<0.05,P<0.01),56mg/kg沸石组与500mg/kg沸石组白细胞数量明显升高(P<0.01);照射后14d,与辐照模型组相比,沸石各给药组小鼠血清中SOD活性显著升高(P<0.05,P<0.01),167mg/kg沸石组与500mg/kg沸石组GSH-Px活性明显升高(P<0.01),56mg/kg沸石组与500mg/kg沸石组骨髓细胞DNA含量明显升高(P<0.05,P<0.01),沸石各给药组骨髓造血干细胞含量明显升高(P<0.01)。结论斜发沸石对亚急性辐射损伤小鼠具有一定保护作用。

Objective To study the protection effect of clinoptilolite（Cp） against radiation injur y. Methods Fortyeight male BALB/c mice were randomly divided into 6 groups, namely normal control group（distilled water ＋ sham irradiation）, radiation control group（distilled water ＋ irradiation）, 300mg/kg 523 group（200mg/kg nilestriol 24 h before irradiation and 100mg/kg nilestriol 4h after irradiation）, 56mg/kg Cp group（56mg/kg Cp ＋ irradiation）, 167mg/kg Cp group（167mg/kg Cp ＋ irradiation） and 500mg/kg Cp group（500mg/kg Cp ＋ irradiation）. Seven days after the administration of the drug, all the mice but those from the normal control group were irradiated with γ-ray irradiation of 137 Cs in the dose of 4.0Gy, at the rate of 0.75Gy/min. All the mice were given the drug for 14 days after irradiation. RBC, WBC and PLT counts in peripheral blood, superoxide dismutases（SOD） activity, malondialdehyde（MDA） level, glutathione peroxidase（GSH-Px） level in blood serum, the content of deoxyribonucleic acid（DNA） and hematopoietic stem cells in bone marrow were determined. Results Compared with radiation control group, on 10 th day after irradiation, RBC counts in peripheral blood of 56mg/kg Cp group and 167mg/kg Cp group were significantly higher（P〈0.05, P〈0.01）, and WBC counts in peripheral blood of 56mg/kg Cp group and 500mg/kg Cp group were significantly higher（P〈0.01）. On 14 th day after irradiation, compared with radiation control group, the SOD activities in blood serum of three Cp groups were elevated（P〈0.05, P〈0.01）, the GSH-Px levels were elevated in blood serum of 167mg/kg Cp group and 500mg/kg Cp group（P〈0.01）, the DNA contents were significantly higher in 56mg/kg Cp group and 500mg/kg Cp group（P〈0.05, P〈0.01）, the hematopoietic stem cells were significantly increased in number in bone marrow of three Cp groups（P〈0.01）. Conclusion The clinoptilolite possesses protective effect against injury induced by 137 Cs γ-irradiation in mice.