不同煎煮时间对解郁顺心汤中无机元素含量的影响

Effects of Different Decocting Time on the Content of Inorganic Element in Jieyu Shunxin Decoction

目的建立电感耦合等离子体原子发射光谱法（ICP-AES）测定解郁顺心汤中不同元素的方法,研究不同的煎煮时间对解郁顺心汤中元素煎出的影响。方法采用HNO3/H2O2微波消解制样,再应用ICP-AES法同时测定解郁顺心汤在煎煮时间为30、60、90min的水煎液中K、Ca、Na、Mg、Al、Zn、Fe、Sn、B、Li、Sr、Cd和As 13种元素的含量组成并进行分析。结果煎煮时间为30min时,解郁顺心汤中的各元素不能够完全析出,煎煮60min各元素基本煎出。解郁顺心汤中含有丰富的元素,其中常量元素中K元素的含量最高,微量元素中Zn元素含量最高。煎煮90min,未检测到Cd和As这2种元素。各元素的线性关系良好,r均大于0.999 0,精密度、稳定性和重复性试验的RSD均符合定量分析要求;加标回收率为95.07%～100.83%,RSD〈3%。结论为了减少煎煮时间且将解郁顺心汤中的各元素尽可能的煎出,煎煮时间应控制在60min。实验结果为解郁顺心汤物质基础和药效学研究提供一定依据。

OBJECTIVE To determine mineral elements content in Jieyu Shunxin Decoction（JYSXD）by ICP-AES,and study the effect of different decocting time on the solution of mineral elements in JYSXD.METHODS Mineral elements（K,Ca,Na,Mg,Al,Zn,Fe,Sn,B,Li,Sr,Cd and As）in JYSXD which was boiled for 30,60,90 min,respectively,were detected by ICP-AES after HNO3/H2O2 digestion.RESULTS After JYSXD was boiled for 30 min,each element was not able to completely precipitated.However,after the decoction was boiled for 60 min,all the elements were completely precipitated.K,Ca,Na,Mg,Al,Zn,Fe,Sn,B,Li and Sr were detected,while Cd and As were not found.Among them,K was the highest in the content of major elements and Zn was the highest in trace elements.Each mineral elements in JYSXD showed good linear relationship（r〉0.999 0）,RSDof precision,stability and repeatability test all satisfied the requirement of quantitative analysis.The recovery was 95.07%～100.83%（RSD〈3%）.CONCLUSION In order to reduce the decocting time and maintain the solution of mineral elements in JYSXD as much as possible,the decocting time should be controlled at 60 min.The experimental results provide basis for material basis and pharmacodynamics study of Jieyu Shunxin Decoction.