小鼠溃疡性结肠炎模型的建立与评价

Establishment and Evaluation of Mouse Model of Ulcerative Colitis

本试验旨在通过筛选葡聚糖硫酸钠盐(DSS)最佳浓度建立小鼠溃疡性结肠炎模型。将30只BALB/c小鼠随机分为对照组、3.5%DSS组、5%DSS组,每组10只。小鼠自由饮水5d,每天记录小鼠体重,观察粪便性状,测便潜血。试验结束后测血清TNF-α、结肠组织髓过氧化物酶(MPO)、丙二醛(MDA)、还原性谷胱甘肽(GSH)活性变化等指标,计算结肠重量/长度比值。与对照组相比,两试验组结肠组织中MPO活性显著升高(P<0.05),MDA含量显著升高(P<0.05),GSH含量显著降低(P<0.05)。结果表明,两种浓度葡聚糖硫酸钠盐均可造模成功,5%DSS组小鼠精神状态很差,表现嗜睡、萎靡状态,而3.5%DSS组小鼠精神状态良好,且组织中MPO、MDA、GSH与对照组相比均差异显著(P<0.05),与5%DSS组相比差异不显著(P>0.05),因此,选用浓度为3.5%DSS造模更合适。

The experiment was aimed to establish mouse model of ulcerative colitis（uc）by screening the optimum concentration of dextran sulfate sodium salt（DSS）.Thirty BALB/c mice were randomly assigned to control group,3.5% DSS group and 5% DSS group,ten mice each group.Mice drank water freely for 5days,the body weights of everyday were recorded,stool was observed and stool occult blood was tested.After the experiment,the changes of TNF-α,MPO,MDA and GSH were tested,and the colon weight/length ratio was calculated.Compared with control group,the activity of MPO and content of MDA in the experiment groups were significantly increased（P〈0.05）,and content of GSH was significantly decreased（P〈0.05）.3.5% and 5%DSS both could successfully establish mouse model of ulcerative colitis.Mice in 5% DSS group had poor mental state,such as lethargy,malaise;Mice in 3.5% DSS group were appropriate,the mice mental was good,MPO,MDA and GSH were significantly different compared with control group（P〈0.05）,but there were no difference compared with 5% DSS group（P〉0.05）.So3.5% DSS was more appropriate than 5% DSS to establish mouse model of ulcerative colitis.