不同底物体外诱导不同基因型红色毛癣菌产生角蛋白酶的活性比较

Comparison of keratinase activity produced by Trichophyton rubrum of different genotypes induced by different substrates in vitro

目的:通过探讨红色毛癣菌体外分泌角蛋白酶的活性与诱导底物及其基因型的关系,从而了解红色毛癣菌的侵袭力与感染部位及其基因型的关系。方法:分别以指甲、趾甲、皮屑和头发为底物,对不同基因型红色毛癣菌T1a和T1b进行体外培养,利用keratin-azure和酶标仪检测其产生的角蛋白酶活性。结果:不同底物诱导红色毛癣菌T1a和T1b产生角蛋白酶活性的高低比较,指(趾)甲高于皮屑(P<0.05),皮屑高于头发(P<0.05),指甲和趾甲之间差异无统计学意义(P>0.05)。同一底物诱导菌株T1a和T1b产生的角蛋白酶活性间差异无统计学意义(P>0.05)。结论:不同底物诱导的角蛋白酶活性不同;红色毛癣菌的侵袭力可能与其感染部位所含的诱导底物有关,而与基因型无关。

Objective： To investigate the relationship between the invasiveness of Trichophyton rubrum and the infection site and the genotype through comparing the activity of keratinase produced by Trichophyton rubrum of different genotypes in- duced by different substrates in vitro. Methods： Two kinds of genotypes of Trichophyton rubrum strains, Tla and Tlb, were cultured in vitro, using human fingernails, toenails, dandruff and hair as substrates respectively. The keratinase extraction was reacted with the keratin-azure and the keratinase activity was detected by the mieroplate reader. Results： Arranged in de- scending order, the keratinase activity of both Tla and Tlb strains from Trichophyton rubrum respectively induced by differ- ent substrates was fingernails, toenails, dander and hair（P〈0.05）, while there was no significant difference between fingernails and toenails （P〉0.05）. There was no significant difference between the keratinase activity of Tla strain and Tlb strain with the same substrate（P〉0.05）. Conclusions： The keratinase activity induced by different substrates is different. The invasiveness of Trichophyton rubrum are probably related to the substrate contained in the infection site, but not to the genotype.