咖啡炭疽病菌生物学特性及其毒力测定

Biological Characteristics and Toxicity Determination of the Colletotrichum gloeosporioides penz from Coffee arabica Linn.

根据形态及ITS序列对咖啡炭疽菌进行了鉴定,其次通过菌丝体生长速率法测定了咖啡炭疽病菌的生物学特性及其毒力。生物学特性测定结果表明：菌丝体生长最适合的温度为28~30℃,p H为7~8。赖氨酸和L-甘氨酸有利于菌丝体的生长,光照及碳源对菌落生长影响则不明显。室内毒力测定结果表明：97.5%腈菌唑对咖啡炭疽病菌的抑菌效果最好,其EC50为30.79μg/m L;其次为95%粉锈宁,其EC_（50）为216.30μg/m L;接着为97.2%百菌清和98%抑霉唑,其EC50分别为305.61、360.77μg/m L。

Abstract The Colletotrichum gloeosporioides penz from coffee was identified by conidium morphology and molecular biology. The biological characteristics and fungicides toxicity were tested by mycelium growth rate. The biological characteristic results showed that the optimal temperature for mycelium growth were between 28℃ and 30℃, the optimal pH values for mycelium growth was from pH7 to pHS, and the optimal nitrogen sources were lysine and leucine. In contrast, illumination and carbon source were insignificant for mycelium growth. The toxicity determination result showed that 97.5% myclobutanil was the greatest inhibited effect on mycelial growth, with the EC50 value of 30.79 μg/mL; following by 95% triadimefon with EC50 value 216.30 μg/mL. Then, 97.2% chlorothalonil and 98% enilconazole has better inhibition effect, the EC50 was 305.61 μg/mL and 360.77 μg/mL, respectively.