强骨抗萎方增强尾吊大鼠承重骨骨矿密度（英文）

Qiang Gu Kang Wei Prescription Increases Bone Mineral Density of Load-bearing Bone in Tail Suspended Rats

目的研究中药复方-强骨抗萎方(QGKWP)对尾吊大鼠骨代谢的影响。方法 21只雄性SD大鼠随机分为对照组(Con),尾吊组(TS)和强骨抗萎方处理组(QGKWP),分别填喂2.5 mL生理盐水或30mg/g中药复方,早晚各1次。每周利用双能X射线吸收测定法检测其骨密度。实验28天后,取血清测定碱性磷酸酶(ALP)、骨碱性磷酸酶(BALP)、骨γ碳氧谷氨酸蛋白(BGLAP)和脱氧吡啶(DPD)。同时检测承重骨力学性能、骨干重和骨灰重。免疫组化检测骨I型胶原(typeⅠcollagen),骨钙素(osteocalcin)和骨桥蛋白(osteopontin)含量。结果与尾吊组相比,强骨抗萎方组股骨、胫骨和腰椎骨密度显著升高,血清ALP和BGLAP表达增加。QGKWP还能明显升高尾吊大鼠股骨I型胶原和骨桥蛋白的表达。结论强骨抗萎方可部分逆转由尾吊引起的失重效应,在对抗骨丢失方面有潜在应用价值。

Objective To investigate the effects of traditional Chinese compound prescription-Qiang Gu Kang Wei Prescription （QGKWP） on bone metabolism of tail suspended rats. Methods Twenty one male SD rats were divided into control group （Con） , the tail suspension WP） randomly. Rats were treated with 2.5 mL saline or 30 group （TS） and QGKWP treatment group （ QGK- mg/g QGKWP by garage every morning and afternoon, respectively. The bone mineral density（BMD） of rats was detected with dual energy X-ray absorptiometry every week. After 28 d of test, blood serum was collected for determination of alkaline phosphatase （ ALP）, bone alkaline phosphatase （ BALP）, bone gamma-carboxyglutamic acid-containing protein （BGLAP） and deoxypyridinoline （DPD）. The mechanical property of weight-bearing bone, bone dry weight and bone ash weight were observed. Collagen I, osteocalcin and osteopontin were detected with immuno-histo-chemistry （IHC） staining. Results Compared with TS, QGKWP significantly increased the BMD of femur, tibia and lumbar vertebrae. QGKWP also promoted serum ALP and BGLAP level. Quantitative IHC analysis showed that QGKWP increased the expression of collagen I and osteopontin in femur of TS rats. Conclusion QGKWP partially reverses the effects induced by TS and may be a potential countermeasure for bone loss.