精子DNA损伤对IVF/ICSI-ET结局影响的回顾性资料分析

Study on sperm DNA fragmentation effecting on the outcomes of In Vitro Fertilization and Embryo Transfer

目的:探讨精子DNA损伤对体外受精/卵胞浆内单精子注射-胚胎移植(IVF/ICSI-ET)结局的影响。方法:回顾性分析了初次行常规IVF和ICSI治疗的192例不孕夫妇的临床资料。分别比较行IVF和ICSI治疗时,精子DNA碎片指数(DFI)≤15%和DFI>15%的受精率、优质胚胎率、种植率、临床妊娠率和流产率的差异;并比较DFI>15%时,不同受精方式(IVF或ICSI)对受精率、优质胚胎率和临床妊娠率的影响。结果:在IVF或ICSI治疗周期中,精子DNA损伤对受精率、优质胚胎率、种植率、临床妊娠率和流产率均无影响(P>0.05)。在DFI>15%情况下,行IVF治疗的受精率、优质胚胎率、种植率和临床妊娠率与行ICSI治疗比较,无统计学差异(P>0.05)。结论:精子DNA损伤不影响IVF/ICSI-ET的结局。对于DFI>15%的患者ICSI-ET与IVF-ET的治疗结局相似。

Objective：To assess sperm DNA fragmentation effecting on the outcomes of In Vitro Fertilization（IVF）and Embryo Transfer（ET）.Methods：Retrospective analysis 192 couples who accepted IVF/Intracytoplasmic sperm injection（ICSI）and ET.According to the DNA fragmentation index（DFI）,the cases were divided into DFI≤15%group（low DFI group）and DFI15% group（high DFI group）.When patients were treated by IVF or ICSI,comparisons were made between high DFI group and low DFI group in the fertilization rate,embryo quality,rate of implantation,rate of clinical pregnancy and rate of abortion.In high DFI group,comparisons were also made between IVF and ICSI in the fertilization rate,embryo quality,and rate of clinical pregnancy rate.Results：During patients were treated by IVF or ICSI,no significant correlation of sperm DFI with fertilization rate,embryo quality,rate of clinical pregnancy and rate of implantation and rate of abortion（P〈0.05）.There was no significant difference when IVF compared to ICSI in the fertilization rate,embryo quality,and rate of clinical pregnancy rate（P〉0.05）.Conclusion：The sperm DNA fragmentation has no any effect on the outcomes of IVF/ICSI and ET.As for patients with DFI≤15%,the outcome are similar when treated by IVF or ICSI.