活血荣络片对AD模型小鼠AchE和ChAT表达的影响

Effect of Huoxue Rongluo Tablets on the Expression of ChAT and AchE in AD Model Mice

目的观察活血荣络片对阿尔茨海默病(AD)小鼠脑组织乙酰胆碱酯酶(Ach E)和乙酰胆碱转移酶(Ch AT)表达的影响。方法将50只APP/PS1双转基因AD模型小鼠随机分为模型组、低剂量活血荣络片组、中剂量活血荣络片组、高剂量活血荣络片组、多奈哌齐组,每组10只。同期将同月龄的10只KM小鼠作为正常组。每天灌胃1次,连续治疗2个月。治疗结束后,取各组小鼠脑组织,采用Western Blot检测Ach E和Ch AT蛋白含量。结果与正常组相比,AD模型组小鼠脑组织Ach E蛋白表达提高、Ch AT蛋白表达降低(P<0.01);与模型组相比,低中高剂量活血荣络片组、多奈哌齐组脑组织Ach E蛋白表达降低、Ch AT蛋白表达增高(P<0.01);与多奈哌齐组比较,中、高剂量活血荣络片组降低Ach E蛋白表达的变化无统计学意义(P>0.05),而高剂量活血荣络片组提高Ch AT蛋白的表达(P<0.05)。结论活血荣络片可一定程度上抑制AD模型小鼠脑组织Ach E蛋白的表达并促进Ch AT蛋白的表达。

Objective： To investigate the effect of Huoxue Rongluo Tablets on the expression of acetylcholinesterase（Ach E） and acetylcholine transferase（Ch AT） in brain tissue of mice with Alzheimer′ s disease（AD）. Methods： 50 APP/PS1 transgenic AD model mice were randomly divided into five groups,with 10 rats in each. 10 KM mice with the same age were chosen as the normal group. The stomach was filled once a day,and continuous treatment lasted for 2 months. After treatment,the brain tissues of mice were taken to detect the content of Ach E and Ch AT with Blot Western. Results： Compared with the normal group,the expression of Ach E protein in brain tissue of mice in AD model group increased,and the expression of Ch AT protein decreased（P 0.01）. Compared with the model group,Ach E protein expression in brain tissue of low,middle and high dose of Huoxue Rongluo Tablets group and donepezil group decreased and the expression of choline acetyltransferase protein increased（P〈 0.01）. Compared with Donepezil group,the reduce of Ach E protein expression in middle and high dose of Huoxue Rong luo decoction group had no statistical significance（P〉0.05）,and high dose of Huoxue Rongluo Tablets group improved Ch AT protein expression（P〈 0.05）. Conclusion： The expression of Ach E protein in brain tissue of AD model mice can be inhibited to a certain extent and Ch AT protein can be promoted with Huoxue Rongluo Tablets.