载高乌甲素壳聚糖纳米粒的制备及其体外释药特性

Preparation of chitosan nanoparticles loaded with lappaconitine and their characteristics of in vitro release

目的制备载高乌甲素壳聚糖纳米粒,并考察其体外释药特性。方法以壳聚糖为载体材料,以三聚磷酸钠为交联剂,采用微乳液-离子交联法制备纳米粒。以包封产率和载药量为主要评价指标,通过正交设计试验优化其制备工艺。结果纳米粒呈球形或类球形,平均粒径为334 nm,在30 h内稳定。高乌甲素的包封产率和载药量分别为(35.34±0.94)%、(2.25±0.08)%。结论该工艺简便可靠,制备的纳米粒具有明显的缓释特征。

AIM To prepare chitosan nanoparticles loaded with lappaconitine and to investigate their characteristics of in vitro release. METHODS The nanoparticles were prepared by microemulsion-ionic crosslinking method,with chitosan as the carrier material and tripolyphosphate as the crosslinker. The encapsulation yield and drug loading rate were taken as main evaluation indices for optimizing the preparation process by orthogonal design test. RESULTS At a mean particle size of 334 nm,the nanoparticles showing spherical or spheroidal shape remained stable within 30 h. The encapsulation yield and drug loading rate of lappaconitine were（ 35. 34 ± 0. 94） %and（ 2. 25 ± 0. 08） %,respectively. CONCLUSION The nanoparticles prepared by this simple and reliable technology can achieve obvious sustained release.