宁夏水稻品种微卫星标记数据库的建立

Establishment of Ningxia Rice Microsatellite Marker Database

本研究以47份经甄别鉴定的宁夏水稻品种（系）为试验材料,筛选出28对较均匀分布于12条染色体、条带清晰稳定、多态性丰富的SSR标记构建了宁夏水稻微卫星标记数据库。共检测到144个等位基因,标记间差异性位点数3~10个,平均5.14个;平均Nei＇s遗传多样性指数为0.6187。28个位点上参试材料间均有差异。只在1个位点上存在差异的是宁粳28号和宁粳23号,宁粳23和宁粳35号;其余品种差异性位点均在2个或2个以上,占参试材料的93.6%。28对引物中筛选出10对核心引物,各材料间至少有1对引物存在差异,能够把参试品种（系）一一区分开来。利用这10对引物,在相同的迁移位置上以1、0标记扩增片段的有无,构建了宁夏水稻DNA指纹图谱。

In this paper,a total of 28 SSR markers distributed on 12 chromosomes were used to construct Ningxia rice microsatellite markers database with 47 varieties which identified carefully. 144 alleles were revealed,and 3 to 10 alleles were identified among different loci. The mean number of alleles per locus was 5. 14. The average Nei genetic diversity index was 0. 6187. The tested material were different within 28 loci. Two pairs of varieties,Ningjing 28 and Ningjing 23,Ningjing 23 and Ningjing 35,which only one locus has difference among loci. While two or more loci have differences among remaining varieties,accounting for 93. 6% to 47 varieties. 10 pairs of core primers were selected from 28 pairs of primers which at least one pair of primers has difference between varieties. In the same position on the migration to 1 and 0 mark represent yes and no to reading data,Ningxia rice DNA fingerprinting database was established using 10 core markers.