朝医三神散对血管性痴呆大鼠学习记忆能力及细胞凋亡的影响

Effects of the Sanshen powder of traditional Korean medicine on learning and memory ability and apoptosis in rats with vascular dementia

[目的]观察朝医三神散对血管性痴呆(VD)大鼠学习记忆能力及海马CA1区神经元细胞超微结构和细胞凋亡的影响.[方法]将实验大鼠随机分为假手术组、模型对照组、喜得镇组、朝医三神散大、中剂量组.采用双侧颈总动脉动脉夹闭方法制备VD模型,应用Morris水迷宫试验检测治疗前后行为学改变,行HE染色观察海马CA1区神经元的结构形态并对神经元数目进行定量分析,应用原位末端标记法(TUNEL)检测细胞凋亡情况.[结果]给予朝医三神散治疗后,大鼠Morris水迷宫逃避潜伏期较模型对照组明显缩短(P<0.05);HE染色观察见,与模型对照组比较,朝医三神散大剂量组细胞排列较整齐且有规律;TUNEL染色观察见,与模型对照组比较,朝医三神散大、中剂量组细胞凋亡个数明显减少(P<0.05).[结论]朝医三神散对VD大鼠学习记忆能力及海马CA1区神经元的细胞结构和细胞凋亡均具有改善作用.

OBJECTIVETo study the effects of the Sanshen powder of traditional Korean medicine on learning and memory ability and ultrastructure and apoptosis of hippocampal neuron CA1 nerve cells in rats with vascular dementia（VD）.METHODSThe rats were randomly divided into groups of sham-operation,model,hydergine tablet,the large and medium dosage of Sanshen powder.Rats models with VD were established by adopting arterial clips to interdict bilateral arteria carotid,and the rats ethological variation before and after treatment was tested by Morris water maze,and morphological change and number of hippocampal neuron CA1 nerve cells were observed by HE staining,and the apoptosis was detected by TUNEL.RESULTS As compared to the model group,the rat＇s escaping latency of Morris water maze after treatment of the Sanshen powder was obviously shortened（P〈0.05）,the hippocampal neuron CA1 nerve cells in the large dosage group were orderliness and regularly arrange,and the number of apoptosis in the large and medium dosage groups was significantly reduced（P〈0.05）.CONCLUSION The Sanshen powder of traditional Korean medicine has improvement effects of learning and memory ability and ultrastructure and apoptosis of hippocampal neuron CA1 nerve cells in rats with VD.