水曲柳FmGT8家族基因生物信息及表达模式分析

Bioinformation and expression pattern analysis of FmGT8 gene family in Fraxinus mandshurica

为研究水曲柳糖基转移酶8家族(glycosyltransferases,FmGT8)基因结构特征及编码蛋白性质,揭示其时空表达模式以及对应拉处理的响应机制,在获得4个水曲柳FmGT8基因的基础上,应用生物信息学软件对Fm GT8及其编码蛋白进行详细的分析;选择5—9月的水曲柳叶子、木质部以及外皮样本,利用实时荧光定量PCR分析FmGT8基因的表达模式;对水曲柳分别进行1、3、7 d的应拉处理,分析Fm GT8基因转录表达对应拉处理的响应模式。结果显示:获得了3个FmGT8全长基因(FmGT8-1,FmGT8-2,FmGT8-3)和1个Fm GT8基因片段(Fm GT8-4),已上传至NCBI并获得登录号分别为KP307672、KP307673、KP307674、KP307675。FmGT8-1、FmGT8-2、FmGT8-3在木质部中表达量最高,Fm GT8-4在外皮中的表达量最高;FmGT8-1、FmGT8-2、FmGT8-3均在6月的相对表达量达到最高,Fm GT8-4在9月表达量最高。4个基因在应拉处理材料中的表达模式各不相同,但均对应拉处理有响应。FmGT8-1、FmGT8-2、FmGT8-4在应拉受力面的表达量最高,FmGT8-3在应压受力面表达量最高,说明FmGT8具有时空特异性。研究结果表明FmGT8家族基因可能参与了细胞壁与木质素的合成。

The structure and characteristics of glycosyltransferase 8 family proteins in Fraxinus mandshurica were studied in this paper. The spatio temporal expression patterns and the response mechanism to gravity were revealed. On the one hand, analysis of structure and temporal expression patterns of FmGT8 genes provide the basis and inspiration for other woody plants ; on the other hand, the study of gene function FmGT8 lay the foundation for directing the transformation of the cell wall composition and wood features and the use of biomass energy. Four FmGT8 genes were cloned from Fraxinus mandshurica in previous research. Application of bioinformation software on FmGT8 and its encoded protein was analyzed in detail. Ash leaf, xylem, skin samples were selected from May to September. The expression pattern in the circadian variation of FmGT8 genes was analyzed by real-time PCR. Tension wood of ash was sampled by one day, three days and seven days treatment. Analysis of the response patterns of FmGT8 gene transcription to gravity treatment. Three full- length FmGT8 genes （FmGT8-1, FmGT8-2, FmGTS-3） and a FmGT8 gene fragment （FmGTS-4） were obtained, which were uploaded to the NCBI and got the accession number （KP307672, KP307673, KP307674 and KP307675）. The expressions of FmGTS-1, FmGT8-2, FmGTS-3 were the highest in xylem, while FmGTS-4 in the skin. The ex- pression of FmGTS-1, FmGT8-2, FmGTS-3 were the highest in June, however, FmGTS-4 expressed in September was highest. The expression patterns of FmGT8 family genes varied in the response to gravity treatment. The expression levels of FmGTS-1, FmGTS-2. FmGTS-4 were the highest in TW, whereas FmGT8-3 in the OW. Expression patterns of FmGT8 family genes were difference which showed that the expressions of FmGT8 genes were spatiotemporal specifici- ty. There were differences among the four genes in response to gravity treatment, which suggested FmGT8 gene family might be involved in cell wall and lignin synthesis.