microRNA-101和COX-2在结肠癌中的表达及其临床意义

Expressions of micro RNA-101 and cyclooxygenase-2 in colorectal cancer

目的:检测结肠癌组织miR-101、COX-2的表达并探讨其临床意义。方法:收集结肠癌组织和癌旁组织标本32例,荧光定量PCR检测miR-101、COX-2 mRNA的表达,分析两者相关性及其与临床病理的关系。结果:与癌旁组织比较,结肠癌组织中miR-101表达相对降低(P<0.01),COX-2 mRNA表达相对增高(P<0.01),且两者表达呈负相关(r=-0.373 5,P<0.01)。结肠癌组织中COX-2的表达与肿瘤的分化程度无关(P>0.05);miR-101随肿瘤分化程度降低其表达也降低(P<0.05)。Dukes分期C^D期肠癌组织中COX-2表达量高于A^B期,miR-101的表达量低于A^B期,但差异均无统计学意义(P>0.05)。结论 :miR-101表达降低及COX-2过表达与结肠癌的发生有关,二者可以成为结肠癌的诊断标志物和潜在治疗靶点。

Objectives To investigate the expressions of miR-101 and COX-2 in colorectal cancer.Methods Thirty-twocolorectal cancer specimens and paired paracancer tissues were collected for assessment of miR-101 and COX-2 expressions by real-time quantitative PCR. Thecorrelation between miR-101 and COX-2, as well as their correlations with the pathologywere analyzed. Results The expression level of mi R-101 and COX-2 mRNA in the cancer tissues was significant difference between the cancer and para-cancer tissues（P 〈0. 01）.Also, miR-101 expression was negatively correlated with COX-2 mRNA expression（r =-0.373 5, P〈 0.01）.Furthermore,miR-101 expression was significantlydecreased along with the deterioration of tumor differentiation grade（P〈 0.05）. Compared with which in Dukes A ～ B stage, COX-2 expression was overexpressed and mi R-101 was down-regulatedin colorectal cancer tissue in Dukes C ～ Dstage, butnosignificant difference was found（ P 〉0.05）. Conclusion Down-regulated miR-101 expression andparallel COX-2 overexpression hasbeen linked to colorectal cancer. miR-101 and COX-2might be thepotentialdiagnostic markers and therapeutic targets.