摘要
试验以小麦为材料 ,纯化了Rubisco活化酶 ,经SDS 聚丙烯凝胶电泳鉴定呈 2条蛋白质谱带 ,分子量为410 0 0和 45 0 0 0 ,利用14 C标记法测定并探讨了Rubisco活化酶的活性 ,并发现Rubisco活化酶在纯化和储藏过程中都需要ATP以稳定其活性。
In this paper, Rubisco activase was separated, purified from wheat leaves and showed two predominant bands at 41 000 and 45 000 presented by SDS-PAGE; Rubisco activase activity was determined and studied by 14C-marking method and it was found that ATP is needed to stabilize its activity during purification and storage.
出处
《中国农业科学》
CAS
CSCD
北大核心
2002年第8期929-933,共5页
Scientia Agricultura Sinica
基金
国家自然科学基金资助项目 ( 39870 46 3)
江苏省自然科学基金项目 (BK2 0 0 114 7)
中国科学技术大学博士生基金项目 ( 99 2 31)
