白腐菌产漆酶活化木粉制备纤维板的力学性能

Mechanical Properties of the Fiberboard from Production of Laccase from White Rot Fungus

利用白腐菌产漆酶的特性,优化连续培养高活漆酶,使得大量培养漆酶成为可能。结果表明:在连续培养和直接培养两种情况下,第11天时漆酶的酶活最高;利用连续培养白腐菌得到的大量的粗漆酶来处理木粉,活化其木质素成分,使其能够替代一部分的脲醛树脂胶制备纤维板。静曲强度上升了18.95%,弹性模量上升了35.49%,内结合强度上升了44.11%。漆酶催化氧化了木质素,增加了木质纤维中化学键的数量,使木质纤维之间的胶结点增多。与此同时,经过漆酶处理的木粉增强了脲醛树脂胶黏剂在纤维表面的渗透和扩散能力,提高了纤维板的力学性能。

By using the characteristics of white rot fungus,we produced laccase,optimized the continuous culture of high activity laccase,and cultivated a large number of laccase. The eleventh day culture showed the highest laccase activity by continuous culture and direct culture methods. Enzyme treatment process can produce environmental protection board,which can solve the problem of formaldehyde pollution caused by indoor decoration. The properties of static bending strength,elastic modulus and interior bond strength were increased by 18.95%,35.49% and 44.11%,respectively. The laccase catalyzed oxidation of lignin,and increased the number of chemical bonds in the fiber so that the bonding points between the wood fibers increased. Wood flour treated by the laccase enhanced urea formaldehyde resin adhesive in fiber surface penetration and diffusion capacity that increased mechanical property of the fiberboard.