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钛合金表面纳米氧化锌薄膜的体外细胞毒性研究

In vitro cytotoxicity study of nano-ZnO thin film on surface of titanium alloy
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摘要 目的评价钛合金表面纳米氧化锌薄膜对细胞毒性的作用。方法观察小鼠胚胎成纤维L-929细胞在含纳米氧化锌薄膜的钛合金析出液中的生长情况,采用噻唑蓝比色法测定各组细胞的吸光度值,并计算出相对的细胞增殖率,进行细胞毒性评价。结果各组细胞生长状况良好,细胞形态无异常,增值率>80%,细胞毒性等级为1级。结论钛合金表面纳米氧化锌薄膜对细胞生长无毒害作用。 Objective To evaluate the cytotoxicity of nano-Zn O thin film on titanium alloy. Methods The growth of L-929 cells in the culture solutions consisting of the ions released from nano-Zn O thin film on the surface of titanium alloy was observed. And the absorbance values of the cells were tested by MTT method.The relative growth rate of L-929 cells was calculated and the cytotoxicity of nano-Zn O thin film on the surface of titanium alloy was evaluated. Results The relative growth rate of each L-929 cell group was above80% and the cytotoxicity grade was grade 1. Conclusions Nano-Zn O thin film on the surface of titanium alloy has almost no cytotoxicity.
出处 《中国现代医学杂志》 CAS 北大核心 2016年第7期1-3,共3页 China Journal of Modern Medicine
基金 中国科学院兰州化学物理研究所固体润滑国家重点实验室开放课题(No:LSL-1309)
关键词 钛合金 纳米氧化锌 细胞毒性 噻唑蓝比色法 生物相容性 titanium alloy nano-ZnO cytotoxicity MTT method biocompatibility
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