基于组学方法的蒙药复方森登-4配伍机制研究

Research on compatibility mechanism of Mongolian medicine Sendeng-4 based on metabonomic methods

目的探究蒙药复方森登-4的配伍机制。方法蒙药文冠木与栀子、川楝子、诃子以5∶3∶1∶1的比例配伍后得到蒙药复方森登-4;配伍前后的样品采用Waters Acquity BEH-C_(18)柱(50 mm×2.1 mm,1.7μm)为色谱柱,0.1%甲酸水溶液-甲醇为流动相进行梯度洗脱,柱温40℃,体积流量0.4 m L/min,进样量10μL;并将超高效液相色谱-质谱联用(UPLC-MS)技术与主成分分析(PCA)、正交偏最小二乘判别分析(OPLS-DA)相结合,对蒙药复方森登-4配伍前后组方中君药文冠木主要化学成分变化情况进行了分析。结果在PCA与OPLS-DA模型中,文冠木与复方森登-4均可明显得到区分;配伍后8个化合物在两组间存在显著差异(P<0.05),其中儿茶素、没食子儿茶素、双氢杨梅素、槲皮素的溶出量在配伍后下降,而xanthocerasic acid、3β-hydroxytirucalla-7,24-dien-21-oic acid、3-oxotirucalla-7,24-dien-21-oic acid、芦丁的溶出量升高。结论蒙药复方森登-4的抗炎及抗风湿活性可能与其中的君药文冠木配伍之后某些指标成分的溶出量变化有一定关系。

Objective To explore the mechanism of the compatibility of Mongolian medicine Sendeng-4. Methods Sendeng-4 was composed of Xanthoceratis Sorbifoliae Lignum seu Ramulus et Folium and its co-decoction with Gardeniae Fructus, Toosendan Fructus, and Chebulae Fructus with a mass ratio of 5 ： 3 ： 1 ： 1. BEH C18 column （50 mm× 2.1 mm, 1.7 mm） was used as the chromatographic column, water （0.1% formic acid）-methanol were used as mobile phase for the gradient elution, and the ultra performance liquid chromatography coupled with mass spectrometry （UPLC-MS）, principle component analysis （PCA）, and orthogonal partial least squares discriminant analysis （OPLS-DA） were used to analyze the chemical constituent changes of Xanthoceratis Sorbifoliae Lignum seu Ramulus et Folium and its co-decoction. Results The significant differences between the group ofXanthoceratis Sorbifoliae Lignum seu Ramulus et Folium and Sendeng-4 were observed in PCA and OPLS-DA model. There were significant differences among eight chemical compounds after compatibility （P 〈 0.05）. The relative contents of catechin, （-）- gallocatechin, dihydromyricetin, quercetin decreased, xanthocerasic acid, 3β-hydroxytirucalla-7,24-dien-21-oic acid, 3-oxotirucalla- 7,24-dien-21βoic acid, and rutin were increased. Conclusion Maybe the anti-inflammatory and anti-rheumatic activity of Sendeng-4have the relationship with the content changes of some index component in Xanthoceratis Sorbifoliae Lignum seu Ramulus et Folium after compatibility.