摘要
建立了一种测定化妆品中EDTA的高效液相色谱方法。试验以含有0.02 mol/L四丁基溴化铵和0.03 mol/L乙酸钠缓冲液4丨丨=4）的乙腈-水（体积比20：80）为流动相，用C18色谱柱对EDTA-Cu络合物进行分离，流速为丨.0 mL/min,检测波长254 nm0结果显示EDTA的检出线性范围为10-200 ng/mL,化妆品中的定量检出限为100 mg/kg,平均回收率为101.7%-1丨7.0%。此方法样品处理简单，分析准确可靠，适用于对各类化妆品中EDTA的检测。
To establish a HPLC method for determination of thecontentofEDTAincosmetic, theEDTA-Cu complexsample was eluted at a flow rate of 1 mL/min using a Cl8 column with mixture of ACN and water （volume ratio 20/80,containing 0.03 mol/L sodium acetate,0.02 mol/L tetrabutyl ammonium bromide, pH=4） as a mobile phase, the wavelength of detection was 254 nm. The linearity was obtained in the range of 10 to 200 ｜ig/mL and the limit of quantification in cosmetic sample was 100 mg/kg, the recovery rate ranged from 101.7% to 117.0%. The method is simple,fast and reliable for determining the content of EDTA in cosmetic samples.
出处
《香料香精化妆品》
CAS
2016年第2期47-51,67,共6页
Flavour Fragrance Cosmetics
基金
上海市质量技术监督局项目资助(编号2014-14)
