一株海洋石油降解菌的特性分析及固定化研究

Characteristics and Immobilization of an Oil-degrading Bacteria Strain

从辽东湾沿岸受石油污染的沉积物中筛选得到一株石油降解菌株BHB-16,对该菌进行了形态以及16SrDNA系统发育分析,初步确定该菌株为Lutibacterium菌属。应用沸石和珊瑚石作为载体进行该菌株的固定化研究,确定当沸石作为载体时,固定化的最佳条件为:菌株接种量为0.6mL,培养时间28h,载体投加量为10mL;选用珊瑚石为载体时,固定化的最佳条件为:菌株接种量为0.6mL,培养时间29h,载体投加量为12mL;此外,用沸石和珊瑚石固定后的菌株,其对于柴油的降解率相对于游离菌分别提高了14.4%和29.6%,初步选用珊瑚石作为载体进行菌株的固定化使其具有更好的石油降解能力。

An oil-degrading strain BHB-16 was isolated from petroleum polluted marine sediments in Liaodong Bay,China.It was identified as Lutibacteriumgenus,according to its morphology and phylogenetic analysis of 16 SrDNA.Then strain BHB-16 was immobilized by zeolite or corlite as the immobilization carrier.The results demonstated that the optimun strain inoculation amount was 0.6mL,incubation time was 28 or 29hours,and dosage of carrier was 10 or 12mL for zeolite or corlite,respectively.The immobilized strain presented better performance in oil degrading than free strain,the oil degradation rate of immobilized strain was 14.4%and 29.6% higher than that of free strain for zeolite or corlite immobilization,respectively.