补肾、活血中药复方对去卵巢骨质疏松症模型大鼠股骨和肾组织IHH mRNA及蛋白表达的影响

Effects of reinforcing kidney and activating blood circulation compound prescription on mRNA and protein expression of IHH in bone and kidney tissues of rats with osteoporosis

目的:观察去卵巢骨质疏松症大鼠模型股骨和肾组织中IHH mRNA和蛋白活性变化,探究中药防治骨质疏松症的分子生物学机制。方法:采用切除雌性SD大鼠双侧卵巢的方式建立骨质疏松症模型,运用补肾中药复方、活血中药复方对模型大鼠进行干预12周,用骨疏康颗粒作为阳性对照药物。RT-PCR检测各组股骨和肾组织IHH mRNA相对表达量;ELISA法检测各组股骨和肾组织IHH蛋白含量。结果:与正常组比较,模型组股骨和肾组织IHH mRNA和蛋白表达量显著减少(P<0.01);与模型组比较,各用药组股骨和肾组织IHH mRNA和蛋白表达均显著增加(P<0.01),且补肾组显著高于活血组(P<0.01)。结论:骨质疏松症的发生机制可能与Hedgehog信号传导通路中IHH含量降低有关;补肾、活血中药复方可以激活IHH信号传导通路,促进骨形成,抑制骨吸收,起到防止骨质疏松症的作用,且补肾中药复方优于活血中药复方。

Objective:To observe the mRNA and protein expression of IHH in bone and kidney tissues of the rats with osteoporosis,and to discuss the molecular biological mechanism of the compound prescription in preventing and treating osteoporosis.Methods:The osteoporosis rat model was established by photosynthesizing the female rats,the osteoporosis rat was treated with experimental compound prescription group for 12 weeks.Gushukang was taken as a positive control drug.Normal control and model control groups were set for comparing.The ELISA and RT-PCR methods were used to investigate the mRNA and protein expression of IHH in bone and kidney tissues.Results:Compared with normal groups,the mRNA and protein expression of IHH in bone and kidney tissues in model control group were significantly decreased(P<0.01).Compared with the model group,the mRNA and protein expression of IHH in bone and kidney tissues in all medicine groups were significantly increased(P<0.01),and the reinforcing kidney group was higher than the activating blood circulation group(P<0.01).Conclusion:The mechanism of osteoporosis may be related to the decrease of IHH content in the Hedgehog signaling pathway.Reinforcing kidney/activating blood circulation compound prescription can activate IHH signaling pathway,promote bone formation,inhibit bone absorption and prevent osteoporosis.Reinforcing kidney compound prescription is superior to activating blood circulation compound prescription compound.