右美托咪定对热射病大鼠血清及外周血单个核细胞HMGB1表达的影响被引量：2

The impact of dexmedetomidine on the expression level of HMGB1 in the serum and PBMCs of heat stroke rats

下载PDF

导出

摘要目的研究右美托咪定对热射病大鼠血清及外周血单个核细胞(PBMCs)中高迁移率族蛋白1(HMGB1)表达的影响及其可能机制。方法 32只SD大鼠分为对照组(C组)、模型组(H组)、右美托咪定组(D组)和育亨宾组(Y组)。麻醉后将大鼠置于(40.0±0.5)℃环境,直至体温达到42℃,建立热射病模型。于热应激终点后1h采血,酶联免疫吸附试验(ELISA)试剂盒测定血清HMGB1浓度,淋巴细胞分离液提取PMBCs,Western blot法测定PBMCs中HMGB1的水平。结果与C组相比,H组大鼠血清HMGB1浓度明显增加,而PBMCs中HMGB1水平明显降低(P<0.05);与H组相比,D组大鼠血清HMGB1浓度降低,而PBMCs中HMGB1水平增加(P<0.05);与D组相比,Y组大鼠血清HMGB1浓度增加,而PBMCs中HMGB1水平降低(P<0.05)。结论右美托咪定降低热射病大鼠血清HMGB1水平,其机制可能与作用于PBMCs表面α2受体,减少其HMGB1的释放有关。 Objective To investigate the impact of dexmedetomidine on the levels of HMGB1 in the serum and PBMCs of heat stroke rats and explore the possible mechanism.Methods Thirty-two rats were divided into four groups（n=8）：control group（group C）,heat stroke model group（group H）,dexmedetomidine group（group D）and yohimbine group（group Y）.Anesthetized rats were subjected to heat stress（40.0±0.5）℃ until the core temperature of the rats reached 42℃to induce heat stroke.The concentration of HMGB1 in the serum was measured by using ELISA kit at 1hafter the heat stress ending.Peripheral blood mononuclear cells（PBMCs）were extracted by using lymphocyte separation medium.The level of HMGB1 in the PBMCs was determined by western blot analysis.Results Compared with group C,the concentration of HMGB1 in the serum of rats in group H was increased significantly（P〈0.05）,and the level of HMGB1 in PBMCs was decreased（P〈0.05）;compared with group H,the concentration of HMGB1 in the serum of rats in group H was decreased significantly（P〈0.05）,and the level of HMGB1 in PBMCs was increased（P〈0.05）;compared with group D,the concentration of HMGB1 in the serum of rats in group Y was increased significantly（P〈0.05）,and the level of HMGB1 in PBMCs was decreased（P〈0.05）.Conclusion Dexmedetomidine could decrease the level of HMGB1 in the serum of heat stroke rats,and the mechanism might be associated with interacting with theα2receptor in the surface of PBMCs and reduced the release of HMGB1 from PBMCs.

作者王珊珊杨晓明王涛

机构地区中国人民解放军空军总医院麻醉科

出处《重庆医学》 CAS 北大核心 2016年第15期2058-2060,共3页 Chongqing medicine

关键词右美托咪定热射病高迁移率族蛋白1 PBMCS dexmedetomidine heat stroke HMGB1 PBMCs

分类号 R614 [医药卫生—麻醉学]

引文网络
相关文献

参考文献21

1Leon LR, Bouchama A. Heat stroke[J]. Compr Physiol,2015,5(2) :611-647.
2Leon LR, Helwig BG. Heat stroke: role of the systemicinflammatory response[J]. J Appl Physiol, 2010,109 (6):1980-1988.
3Andersson U,Tracey KJ. HMGB1 is a therapeutic targetfor sterile inflammation and infection[J]. Annu Rev Im-munol, 2011,29 : 139-162.
4Dehbi M,Uzzaman T,Baturcam E,et al. Toll-like receptor4 and high-mobility group box 1 are critical mediators oftissue injury and survival in a mouse model for heatstroke[J]. PLoS One,2012,7(9) :e44100.
5Tong HS, Tang YQ, Chen Y, et al. Early elevated HMGB1level predicting the outcome in exertional heatstroke [J]. JTrauma,2011,71(4) :808-814.
6Hagiwara S,Iwasaka H, Goto K, et al. Recombinant throm-bomodulin prevents heatstroke by inhibition of high-mobilitygroup box 1 protein in sera of rats[J]. Shock, 2010,34(4):402-406.
7Pandharipande PP,Sanders RD,Girard TD,et al. Effect ofdexmedetomidine versus lorazepam on outcome in patientswith sepsis: an a priori-designed analysis of the MENDSrandomized controlled trial[J]. Crit Care,2010,14 (2):R38.
8Gu J, Sun P, Zhao H, et al. Dexmedetomidine providesrenoprotection against ischemia-reperfusion injury in mice[J]. Crit Care,2011,15(3) :R153.
9Xu L, Bao H, Si Y, et al. Effects of dexmedetomidine onearly and late cytokines during polymicrobial sepsis inmice[J]. Inflamm Res,2013,62(5) : 507-514.
10Chen GM, Xu HN, Gao LF,et al. Effects of continuoushaemofiltration on serum enzyme concentrations,endo-toxemia,homeostasis and survival in dogs with severeheat stroke[J]. Resuscitation, 2012,83(5) : 657-662.