利用元素分析仪测定面粉中粗蛋白方法的研究

Determination of crude protein content in wheat fl our by the elemental analyzer method

探讨元素分析仪测定面粉中粗蛋白方法的可行性。利用元素分析仪测定面粉中的粗蛋白含量,与传统的凯氏定氮法作对比,研究元素分析法的准确性和精密度,并建立了2种方法之间的回归模型。研究结果表明,元素分析仪的测定结果接近凯氏定氮仪,相对误差小于5%,RSD在0.317%~1.99%范围内。元素分析法与凯氏定氮法间的回归预测方程为y=0.98916x-0.05236(R^2=0.98018),元素分析法可以较好地预测凯氏定氮法的测定结果。元素分析仪测定面粉中的粗蛋白准确度和精密度较好,是一种快速检测面粉中粗蛋白含量的方法。

This paper discussed the feasibility of the elemental analyzer for the determination of crude protein in wheat flour. The precision and accuracy of the elemental analyzer was discussed by determining of crude protein content in flour by element analyzer, and compared with the traditional Kjeldahl method. The regression model between the two methods was also established. The results showed that the determination result of elemental analyzer was near the Kjeldahl apparatus, the relative error of which was less than 5%. RSD of elemental analyzer was in the range of 0.317%-1.99%. The regression equation between the elemental analysis method and Kjeldahl nitrogen method was y=0.98916x-0.05236 （R2=0.98018）, which could predict the result of the Kjeldahl nitrogen determination method well. Element analyzer method for determination of crude protein in wheat flour was suitable for rapid detection of crude protein content in wheat flour with high accuracy and precision.