实时荧光定量PCR对川贝母的鉴别应用

Application of Real-time Fluorescent Quantitative PCR in the Identification of Fritillariae Cirrhosae Bulbus

目的:探讨实时荧光定量PCR对川贝母鉴别的可行性。方法:采用磁珠法从试样中提取DNA,利用实时荧光定量PCR进行引物扩增,并通过其Cq值和扩增曲线对样品进行判断。结果:5批川贝母的Cq值均低于30,曲线有明显的指数增长期;平贝母、浙贝母和土贝母则无Cq值,曲线为一直线。结论:本方法用于鉴别川贝母操作简单,准确率高,重现性好,可行有效。

Objective： To investigate the feasibility of real-time fluorescence quantitative PCR in the identification of Fritillariae Cirrhosae Bulbus. Methods： The DNA of samples was extracted by magnetic beads, the primers were amplified by real-time fluores- cence quantitative PCR, and the Cq value and amplification curve were used to determine the samples. Results： The Cq values for five batches of Fritillariae Cirrhosae Bulbus were lower than 30, and the curve had obvious growth period. No Cq value was shown for Fritil- lariae Ussuriensis Bulbus, Fritillariae Thunbergii Bulbus and Bolbostemmatis Rhizoma with straight line curves. Conclusion： The meth- od is simple.feasible and effective in the identification of Bulbus FritiUariae Cirrhosae with high accuracy and good reproducibility.