低温处理对白三叶叶绿素、可溶性糖和丙二醛等的影响

Effects of Temperature Stress on Chlorophyll Content, Soluble Sugar and MDA in Trifolium repens L.

为探索低温胁迫对白三叶的叶绿素、可溶性糖和丙二醛(MDA)等生理指标的影响,试验以新品系‘08-2’和‘海法’白三叶(Trifolium repens L.‘haifa’)为材料,依次设置10、5、0、-5、-10、-15℃处理,研究低温下叶绿素、可溶性糖和MDA等的变化,对材料的抗寒性进行评价。结果表明,变温对白三叶的叶绿素、可溶性糖和MDA等的影响较大。随着温度的降低,白三叶的叶绿素含量总体呈现降低的趋势。在较低(≤0℃)温度处理下,‘08-2’的叶绿素含量降低速度小于‘海法’的;‘08-2’的可溶性糖含量在0℃时达到最高,‘海法’的在5℃达到最高;随着温度的降低,MDA含量和电导率逐渐升高,但‘08-2’变化幅度低于‘海法’。综合分析表明,新品系‘08-2’耐寒性优于‘海法’白三叶。

The objective of this study is to explore the effect of low temperature stress on chlorophyll, soluble sugar and MDA in Trifolium repens L.. The new strain‘08-2＇and‘Haifa＇of Trifolium repens were used as the materials, and then the treatments of 10, 5, 0,-5,-10,-15℃ were set. The variations of chlorophyll, soluble sugar and MDA were studied under low temperatures, and the cold resistances of the materials were evaluated.The results showed that varying temperature had a great influence on chlorophyll, soluble sugar and MDA in Trifolium repens L.. With the decrease of temperature, the chlorophyll content declined gradually. The declining speed of‘08-2＇was less than that of‘Haifa＇under lower temperature（≤0℃）. The soluble sugar content of‘08-2＇was the highest at 0℃, and the soluble sugar content of‘Haifa＇was the highest at 5℃. With the decrease of temperature, the MDA content and electric conductivity increased gradually, but the change range of‘08-2＇was lower than that of‘Haifa＇. Comprehensive analysis showed that the cold resistance of the new strain‘08-2＇was better than that of‘Haifa＇.