红细胞感染附红细胞体后的免疫因子变化分析

Analysis on the Changes of Immune Factors after Red blood Cells Infected with Eperythrozoonosis

目的研究小鼠的红细胞感染附红细胞体后其红细胞的相关免疫因子变化。方法使用带有阳性猪附红细胞体的血液,在小鼠体内建造红细胞感染模型;在第1、2、4、6、8日取血,采用酶联免疫吸附试验方法检测小鼠体内CD35、CD58和CD59水平。结果感染组小鼠在感染附红细胞体后第1、2、4、6、8日,红细胞表面的CD35水平呈下降趋势[(55.19±0.03)%、(54.91±0.06)%、(53.87±0.06)%、(54.18±0.06)%、(54.61±0.07)%],对照组无变化[(55.68±0.04)%、(55.66±0.05)%、(55.67±0.06)%、(55.68±0.05)%、(55.67±0.05)%],两组在组间、不同时点间、组间·不同时点间交互作用差异有统计学意义(P<0.05);在第1、2、4、6、8日血浆CD35[(55.39±0.02)%、(58.99±0.63)%、(58.01±0.05)%、(55.46±0.04)%、(54.92±0.03)],CD58[(17.79±0.03)%、(22.87±0.05)%、(20.91±0.04)%、(18.19±0.03)%、(17.33±0.01)],CD59[(17.19±0.02)%、(22.87±0.05)%、(21.07±0.05)%、(17.58±0.03)%、(17.31±0.02)]水平呈现上升到达峰值后逐渐下降的趋势,在第2日达到最高值,对照组CD35[(54.08±0.03)%、(54.11±0.02)%、(54.11±0.03)%、(54.09±0.02)%、(54.11±0.04)%]、CD58[(17.15±0.05)%、(17.15±0.03)%、(17.17±0.02)%、(17.13±0.04)%、(17.16±0.01)%]、CD59[(16.86±0.01)%、(16.81±0.09)%、(16.83±0.05)%、(16.85±0.04)%、(16.85±0.03)%]无变化,两组在组间、不同时点间、组间·不同时点间交互作用差异有统计学意义(P<0.05)。结论小鼠在感染附红细胞体后,红细胞表面的CD35、CD58、CD59水平下降,红细胞的免疫功能受到一定的影响,严重时可能影响机体整个免疫系统的功能。

Objective To study the changes of erythrocyte-associated immune factors after erythrocytes being infected with eperythmzoonosis in mice. Methods The positive suis blood was used to construct eryth- rocyte infection model in mice, and the ELISA method to detect mouse CD35, CDss and CD59 levels on the lst,2nd,4th,6th,Sth day after injection. Results After infected with eperythrozoonosis,on the lst,2nd, 4th ,6th,8th day CD35 on the surface of the red blood cell levels dropped [ （ 55.19 ± 0. 03 ） %, （ 54.91 ± 0.06） %, （53.87 ± 0. 06 ） %, （ 54. 18 ± 0. 06 ） %, （ 54. 61 ± 0. 07 ） % ], no change in the control group [ （55.68 ±0.04）% ,（55.66 ±0.05）%, （55.67 ±0.06）%, （55.68 ±0. 05）%, （55.67 ±0.05）% ], there were significant differences between the two groups, the different time points and the interaction of groups ± time points （P 〈 0. 05 ）. On the lst,2nd,4th,6th, 8th day serum CD35 of the infected group [ （55.39 ±0.02）%,（58.99 ±0.63）% ,（58.01±0.05）% ,（55.46 ±0.04）% ,（54.92 ±0.03） ] ,CD58 [ （17.79 ±0. 03）%, （22.87 ±0. 05）%, （20. 91±0. 04）%, （18. 19 ±0. 03）%, （17.33 ±0. 01 ） ] ,CD59 [ （17. 19 ±0. 02）% ,（22. 87 ±0.05）% ,（21.07 ±0.05）% ,（17.58±0. 03）% ,（17.31 ±0.02） ] levels fist increased and then decreased,the peak level appeared on the 2nd,of the control group CD35 [ （54. 08 ± 0.03）% ,（54. 11 ±0.02）% ,（54. 11 ±0.03）%, （54. 09 ±0.02）% ,（54. 11 ±0.04）% ] ,CDss [ （17. 15 ± 0.05）% ,（17. 15 ±0.03）% ,（17. 17 ±0.02）% ,（17. 13 ±0.04）% ,（17. 16 ±0.01）% ] ,CD59± （16.86 ± 0. 01）% ,（16. 81 ±0.09）% ,（16. 83 ±0. 05）% ,（16. 85 ±0. 04）% ,（16. 85 ±0.03）% ] there were no sig- nificant changes, there were significant differences between the two groups, the different time pointss and the interaction of the groups time points （ P 〈 0. 05 ）. Conclusion In the mice infected eperythrozoonosis, CD35, CD58, CD59 of red blood cell surface decrease, and the immune function of red blood cells is impaired to some degree, which may affect the function of the entire immune system in serious cases.