黄芪粗提物对小鼠急性酒精性肝损伤的保护作用

Treatment effect of Astragalus extract on acute alcoholic liver injury in mice

通过连续灌胃酒精建立小鼠急性酒精性肝损伤模型,研究黄芪粗提物对小鼠肝脏的保护作用。设空白组、模型组、阳性组和黄芪粗提物高、中、低剂量组(8、4、2g/kg),分别检测肝脏指数,血清中丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)以及肝脏中总胆固醇(TC)和甘油三酯(TG)的含量,采用HE染色观察肝脏损伤和脂肪变性情况。结果表明:黄芪粗提物下调肝脏指数,减少血清中ALT、AST以及肝脏中TC、TG的分泌,抑制肝脏损伤和脂肪变性形成,与模型组比较,黄芪粗提物高剂量组具有极显著差异(P<0.01),黄芪粗提物中、低剂量组具有显著差异(P<0.05)。黄芪粗提物对小鼠急性酒精性肝损伤有保护作用,可为黄芪粗提物在临床上的应用提供理论指导。

The model of acute alcoholic liver injury in mice was established by giving alcohol continuously,the protective effect of Astragalus extract on the liver of mice was investigated.The mice were divided into blank group,model group,positive group and Astragalus extract high,medium and low dose groups（8,4,2g/kg）.The liver index was determined,the content of ALT and AST in serum was investigated,the content of TC and AST in liver was determined.The degree of hepatic steatosis was determined using HE staining.The result showed that Astragalus extract could treat acute alcoholic liver injury by abating liver index,reducing the secretion of AST and AST in serum and the secretion of TC and TG in liver of mice,inhibiting the injury of liver and the form of hepatic steatosis.Compared with model group,Astragalus extract high dose group had very significant difference（P〈0.01）,medium and low dose groups had significant difference（P〈0.05）.It suggests that Astragalus extract has certain protective effect on acute alcoholic liver injury of mice and provides a theoretical guidance.