油梨果渣化学成分分析及其多酚类成分的清除自由基能力

Phenolic Compounds and Their Capacity of Scavenging Free Radicals from Residues in Pulps of Persea americana

目的:对油梨果渣化学成分进行分离和结构鉴定,并对多酚类化合物体外清除自由基能力进行评价。方法:采用常压柱色谱和高压液相制备色谱相结合的分离方法对油梨果渣进行化学成分分离纯化,通过对波谱数据分析和文献比对进行结构鉴定;并以1,1-二苯基-2-三硝基苯肼(DPPH·)自由基清除法体外评价单体化合物清除自由基能力。结果:从油梨果渣中分离得到7个化合物,分别鉴定为1,2,4-trihydroxyheptadec-16-yne(1),1,2,4-trihydroxynonadecane(2),(2R,12Z,15Z)-2-hydroxy-4-oxoheneicosa-12,15-dien^(-1)-yl acetate(3),色氨酸(4),绿原酸(5),新绿原酸(6),东莨菪苷(7);体外清除自由基活性实验表明化合物5和6清除自由基能力与维生素C相近。结论:化合物4,6,7首次从油梨果肉中发现,并且分离得到的多酚类化合物具有较好的清除自由基活性。

Objective： To isolate and identify the chemical compounds from the residues in the pulps of Persea americana, and evaluate the capacity of scavenging free radicals of phenolic compounds. Method： The chemical compounds were isolated and purified by the combined normal pressure column chromatography and high pressure liquid preparative chromatography, and their chemical structures were elucidated by spectral data analysis and comparing with the previous literature. Moreover, the capacity of scavenging free radicals of phenolic compounds was also evaluated by using 1, 1 diphenyl - 2 - trinitrobenzene hydrazine （DPPH＇） assay. Result： Seven compounds were obtained and their structures were identified as 1, 2, 4-trihydroxyheptadec-16-yne （1） , 1, 2, 4-trihydroxynonadecane （ 2 ） , （ 2R, 12Z, 15Z） -2-hydroxy-4-oxoheneicosa-12, 15-dien-l-yl acetate （3）, tryptophan （4）, chlorogenic acid （5）, neochlorogenic acid （6）, and scopolin （7） respectively. The in vitro free radicals scavenging activity experiment showed that the capacity of scavenging free radicals of compounds 5 and 6 were close to that of vitamin C. Conclusion： Compounds 4, 6 and 7 were isolated from P. americana for the first time. In addition, phenolic compounds obtained from P. americana had good in vitro free radicals scavenging activities