摘要
为了解Ⅰ群腺病毒(FAV-Ⅰ)在鸡群中的感染状况,对收集的178份疑似病料进行鸡胚病变、细胞病变特征分析,利用RT-PCR、基因测序和BLAST进行病毒的分离鉴定,成功分离出25株FAV-Ⅰ,其中20株与血清4型同源性较高,5株与血清8型同源性较高。动物回归试验结果表明,攻毒鸡的临床症状和病理变化与自然发病鸡基本一致。
In order to evaluate infection status of fowl adenovirus group I （FAV-I） in chickens, characteristic analysis of embryo lesion and cytopathic effect was carried out towards the collected 178 suspected samples.Virus was isolated and identified by RT-PCR, gene sequencing and BLAST test, and 25 strains of FAV-I were successfully isolated. Among all of them, 20 strains have high homology with serotype 4, and 5 strains have high homology with serotype 8. Results of animal regression test indicated that the clinical symptoms and necropsy lesions of challenging chickens were consistent with natural infection.
出处
《中国动物检疫》
CAS
2016年第8期86-89,97,共5页
China Animal Health Inspection
关键词
Ⅰ群禽腺病毒
分离
鉴定
血清型
致病性
fowl adenovirus group I
isolation
identification
serotypes
pathogenicity
