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人尿源干细胞移植治疗慢性肾病大鼠 被引量:12

Human urine-derived stem cell transplantation for the treatment of chronic kidney disease in rats
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摘要 背景:尿源干细胞最可能的来源是肾脏组织,可能更加适应肾脏微环境,这为肾脏疾病的治疗提供了新思路。目的:探讨人尿源干细胞对慢性肾病模型大鼠的治疗作用。方法:收集健康人新鲜尿液,提取人尿源干细胞,进行体外培养。20只SD大鼠制备慢性肾病模型,实验组肾皮质内注射人尿源干细胞悬液,对照组肾皮质内注射生理盐水,另外取10只健康大鼠作为正常组。通过检测3组大鼠血清肌酐及肾小球滤过率判断尿源干细胞对肾功能的改善作用,并对3组大鼠肾脏进行组织形态学评估。结果与结论:(1)尿源干细胞对肾功能具有改善作用,与对照组比较,实验组大鼠血清肌酐降低、肾小球滤过率升高;(2)尿源干细胞对肾脏的组织学形态有改善作用,与对照组比较,实验组大鼠肾脏组织中CD68阳性细胞明显减少,肾脏间质炎性细胞浸润减轻;(3)结果表明,人尿源干细胞对慢性肾病模型大鼠肾功能存在修复作用。 BACKGROUND: Urine-derived stem cells are most likely to come from the kidney tissue, and therefore,these cells are more adaptable to kidney microenvironment, providing a new option for the treatment of kidney diseases.OBJECTIVE: To explore the therapeutic efficacy of human urine-derived stem cells on chronic nephropathy rats.METHODS: The fresh urine samples of healthy people were collected, and then human urine-derived stem cells were extracted and cultured in vitro. Twenty Sprague-Dawley rats were used to prepare chronic nephropathy models, and given injection of human urine-derived stem cell suspension(experimental) or normal saline(control) into the renal cortex, respectively. Another 10 healthy rats were used as controls.Therapeutic effects on renal function were assessed by detection of serum creatinine level and glomerular filtration rate in the three groups. The kidney tissues of rats were taken and observed histomorphologically in each group.RESULTS AND CONCLUSION: Human urine-derived stem cells were found to remarkable improve rat's renal function as well as reduce the histomorphological changes in the kidney tissues of rats. Compared with the control group, the serum creatinine level was decreased while the glomerular filtration rate was increased significantly in the experimental group; CD68 expression and infiltration of interstitial inflammatory cells were also markedly reduced in the experimental group. To conclude, human urine-derived stem cells can improve the renal function of chronic nephropathy rats.
出处 《中国组织工程研究》 CAS 北大核心 2016年第32期4838-4844,共7页 Chinese Journal of Tissue Engineering Research
基金 河北省医学科学研究重点课题计划指令性课题(ZL20140062)~~
关键词 肾病 尿 干细胞移植 组织工程 干细胞 移植 人尿源干细胞 慢性肾病 大鼠 血清肌酐 肾小球滤过率 CD68 Nephrosis Urine Stem Cell Transplantation Tissue Engineering
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