扁蒴藤素对紫杉醇耐药乳腺癌细胞生长的抑制作用

Inhibitory Effect of Pristimerin on Growth of Taxol-Resistant Breast Cancer Cells

目的:研究扁蒴藤素对紫杉醇耐药乳腺癌细胞MCF-7/TAX生长的作用。方法:以乳腺癌细胞株MCF-7为对照,同时制作紫杉醇耐药乳腺癌细胞MCF-7/TAX;在两组细胞中分别给与7种浓度(0.25、0.5、1、2.5、5、10和20μmol/L)扁蒴藤素或紫杉醇培养48 h,用MTT法检测药物对MCF-7和MCF-7/TAX细胞作用的半数抑制浓度(IC50)值,并计算MCF-7/TAX细胞对紫杉醇或扁蒴藤素的耐药指数(RI);同时用Annexin VFITC/PI染色观察扁蒴藤素作用后MCF-7/TAX凋亡细胞比例。结果:MCF-7/TAX对紫杉醇RI达19.39,而扁蒴藤素对MCF-7/TAX细胞的生长有高效抑制作用,且呈现明显的剂量依赖性,对其作用48 h的IC50为0.52μmol/L,MCF-7/TAX对扁蒴藤素的RI仅为0.88;扁蒴藤素作用后,Annexin V染色阳性的MCF-7/TAX细胞比例较对照明显升高。结论:扁蒴藤素能抵抗紫杉醇耐药乳腺癌细胞的耐药性,激活MCF-7/TAX细胞凋亡,对其生长发挥高效抑制作用。

Objective： To investigate the effect of pristimerin on the growth of taxol-resistant breast cancer cells MCF-7/TAX. Methods： The breast cancer cell line MCF-7 was the control group, and the taxol-resistant breast cancer cells line MCF-7/TAX was the observation group. In two groups of cells were cultured in 7 kinds of concentrations （0.25, 0.5, 1, 2.5, 5, 10 and 20 tool/L） pristime- rin and paclitaxel for 48 h. MTT assay was used to detect half inhibition concentration （IC50） value of drug on MCF-7 and MCF-7/tax ceils, and resistance index （RI） of cells to paclitaxel or pristimerin were calculated. At the same time Annexin V-FITC/PI staining was adopted to observe changes of pro- portion of apoptotie cells after pristimerin involvement. Results： The resistance index of MCF-7/TAX ceils to paclitaxel was 19.39. After treatment with pristimerin for 48 h, MCF-7/TAX ceils displayed markedly growth inhibition in a dose-dependent manner, and ICS0 for 48 h was 0.52 μM. The resist- ance index of MCF-7/TAX cells to pristimerin was 0. 88. After treatment with pristimerin, the propor- tion of positive MCF-7/TAX cells of Annexin V-FITC/PI staining was significantly higher than that of the control group. Conclusion： Pristimerin can resist resistance to paclitaxel resistance to breast canc-er cells, activate apoptosis of MCF-7/TAX cells, thus effectively inhibit cancer cells growth.