芍药苷对哮喘模型小鼠气道炎症趋化因子及受体的干预作用

Inhibitory effect of paeoniflorin on inflammatory chemokines and their receptor in a mouse model of asthma

目的观察芍药苷给药后对小鼠哮喘模型气道炎症趋化因子及受体的影响。方法用卵蛋白(OVA)致敏和激发建立小鼠哮喘模型;ELISA法检测血清IL-6、TNF-α水平及支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中卵蛋白特异性Ig E(OVA-Ig E)和趋化因子CCL19、CCL21水平;RT-PCR法检测肺组织中趋化因子受体CCR7mRNA表达;Western blot检测肺组织CCR7及核转录因子-κB(nuclear factor-κB,NF-κB)蛋白表达。结果芍药苷干预组小鼠血清IL-6、TNF-α水平显著下降;BALF中OVA-Ig E和CCL19、CCL21水平显著降低;肺组织CCR7mRNA、CCR7及NF-κB蛋白表达明显减少。结论芍药苷对哮喘模型小鼠气道炎症趋化因子CCL19/CCL21及其受体CCR7具有显著的抑制作用。

Objective To study the effect of paeoniflorin on inflammatory chemokines and their receptor in a rat model of ovalbumin-induced asthma. Methods Sixty 6-week old SPF female BALB/c mice were used in this study. To establish a mouse model of asthma by sensitizing and challenging with ovalbumin. ELISA was used to analyze the serum IL-6and TNF-α,and the specific Ig E against ovalbumin（ OVA-Ig E）,CCL19 and CCL21 in bronchoalveolar lavage fluid（ BALF）. RT-PCR was performed to determine CCR7 mRNA and protein expression of chemokine receptor CCR7,and the level of NF-κB was tested by Western blot. Results In In the paeoniflorin groups,the serum IL-6 and TNF-α levels were significantly lower,and the OVA-Ig E,CCL19 and CCL21 levels in BALF were significantly reduced,compared with that in the control group. CCR7 mRNA and protein expression of chemokine receptor CCR7 and NF-κB in the lung were significantly reduced by paeoniflorin. Conclusions Paeoniflorin has a remarkably inhibitory effect on the airway inflammatory chemokines CCL19/CCL21 and the receptor CCR7 in the mouse model of asthma.