粳稻品种Oochikara稻瘟病抗性基因挖掘

Rice blast-resistant gene mining in japonica rice cultivar Oochikara

对水稻品种Oochikara(Oryza sativa L.ssp.japonica)进行稻瘟病抗性分析,发现Oochikara对来自不同地区的30个稻瘟菌菌株具有高效的广谱抗性。利用等位基因挖掘技术,从Oochikara鉴定出4个已知稻瘟病抗性基因(R gene):Pish、Pia、Pikm和Pita。此外,利用稻瘟菌菌株08-7-3对Oochikara和感病品种E103杂交后代进行稻瘟菌喷雾接种和遗传分析,发现Oochikara还存在其他新的抗性基因。以上研究结果初步揭示了Oochikara对稻瘟病的抗性来源,有助于利用Oochikara作为抗性中间材料开展稻瘟病抗性育种工作。

A rice japonica cultivar Oochikara confers broad spectrum resistance to thirty Magnaporthe grisea strains collected from different regions. Four known rice blast resistance genes （ R gene） , Pish,Pia,Pikm and Pita were identified from Oochikara by allele mining approach. Moreover, genetic analysis and inoculation with the M. grisea strain 08-7-3 by spraying were performed in the progenies derived from a cross of Oochikara and a blast susceptible cultivar E103 showed that there were some other novel R gene （ s ） in Oochikara. The findings revealed the source of rice blast resistance in Oochikara, which will facilitate the development of elite cultivars against rice blast using Oochikara as an intermediate ma-terial for breeding.