纯钛表面淫羊藿苷/TiO_2纳米管复合涂层制备与药物早期释放的分析

Titanium dioxide nanotube composite coating: preparation and early drug-release

背景:对纯钛进行表面改性,提高钛种植体早期骨结合能力是目前研究的热点。目的:在TiO_2纳米管表面加载淫羊藿苷形成复合涂层,研究其载药量及早期释放规律。方法:通过阳极氧化在光滑纯钛表面形成TiO_2纳米管,用扫描电镜、原子力显微镜、接触角测定仪对光滑纯钛及TiO_2纳米管进行表征。通过浸泡法在纯钛及TiO_2纳米管表面加载淫羊藿苷,使用高效液相色谱法测定两种材料的早期药物释放量,计算出载药量,绘制累积释放量曲线和累计药物释放量百分比曲线。结果与结论:(1)TiO_2纳米管径为80 nm,其粗糙度大于纯钛(P<0.05),接触角小于纯钛(P<0.05);(2)淫羊藿苷/TiO_2纳米管组前14 d的累计药物释放量和前4 h的药物累积释放曲线明显高于淫羊藿苷/纯钛组;与淫羊藿苷/纯钛组相比,淫羊藿苷/TiO_2纳米管组累计药物释放量百分比曲线更为平缓,释放时间更长;(3)结果表明,淫羊藿苷/TiO_2纳米管复合涂层可提供较高的药物加载量并具有缓释功能。

BACKGROUND： The surface modification of pure titanium has become a hotspot for research on improving the early implant-osseointegration ability. OBJECTIVE： To construct the icadin/TiO2 nanotube composite coating, and to explore its drug-loading rate and early drug-release kinetics. METHODS： Pure titanium was anodized to obtain TiO2 nanotube. Thereafter, the pure titanium and TiO2 nanotube ware characterized by scanning electron microscopy, atomic force microscopy and contact angle instrument. Icariin was loaded onto the pure titanium and TiO2 nanotube by immersion method. Afterwards, the early drug-releasing amount of the two materials was measured using high-performance liquid chromatography. Accumulated release and accumulated drug-release percentage curves were drawn. RESULTS AND CONCLUSION： The diameter of TiO2 nanotube was 80 nm, and the roughness of TiO2 nanotube was significantly higher than that of pure titanium （P 〈 0.05）, but the contact angle was significantly lower than that of pure titanium （P 〈 0.05）. Moreover, compared with the icariin/pure titanium group, the icariinFFiO2 nanotube group had obviously higher accumulated drug-releasing amount at former 14 days and accumulated drug-releasing carve at former 4 hours. The accumulated drug-releasing percentage curve in the icariin/TiO2 nanotube group was flatter than that in the icariin/pure titanium group, suggesting a longer release time. To conclude, the icariin/TiO2 nanotube composite coating has a higher drug, loading and exerts better sustained-release effect.