狗枣猕猴桃多酚对^60Coγ射线辐射防护作用的研究

The radioprotection of Actinidia kolomikta polyphenols against organism damage by ^(60)Co γ- rays

以狗枣猕猴桃为原料,研究分离纯化的狗枣猕猴桃多酚(AKP)的辐射防护作用。雄性昆明小鼠随机分为6组:正常对照组、辐射模型组、利可君片组(1.4 mg/kg·d)、狗枣猕猴桃多酚低、中、高剂量组(分别为50、100、200 mg/kg·d),连续给药14 d,除正常对照组外所有小鼠采用^(60)Coγ射线进行全身照射,剂量率为0.792 Gy/min,辐射剂量为6.0 Gy,辐射后观察小鼠体内抗氧化物酶以及丙二醛(MDA)的表达情况,同时测定还原型谷胱甘肽(GSH)的含量。结果表明:与对照组相比,模型组小鼠各组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性降低(p<0.05),MDA含量升高(p<0.01)。与模型组相比,中剂量的AKP能够显著提高辐射小鼠体内SOD的活性(p<0.05);低剂量AKP能够使心脏、肾脏、血浆中CAT活性基本恢复到正常对照组水平;中剂量AKP使肝脏、脾脏、血浆中GSH-Px活性显著提高(p<0.01),不同剂量的AKP能够有效降低辐射小鼠各组织中MDA含量(p<0.05);中剂量的AKP使机体各组织中乳酸脱氢酶(LDH)的活性基本恢复到正常水平。但是总体效果不及利可君片。因此,狗枣猕猴桃多酚可一定程度地保护机体,减少氧化损伤对机体造成的不良影响,也为其进一步开发利用提供了科学依据。

In this paper selected Actinidia kolomikta as raw material, to investigate radiation-protective effect of Actinidia kolomikta polyphenols （AKP） by separation and purification. Male Kunming mice were divided into 6 groups randomly, control group, radiation model group, leucogen tablets group, low AKP group （50 mg/mg/kg· d）, medium AKP group （ 100 mg/mg/kg · d）, and high AKP group （：200 mg/mg/kg ·d）. The interventions for AKP exposure continued for 14 consecutive days.Except contrast group, all .5 groups were irradiated by ^60Coγ-rays, the dose rate was 0.792 Gy/min,and radiation dose was 6.0 Gy.After radiation, analyze expression of antioxidant enzymes, MDA and GSH in mice.The results indicated that model mice significantly decreased in tissue SOD, CAT, GSH- Px and GSH content （ p 〈 0.05 ）, increased MDA content （ p 〈 0.01 ）, compared with control group.Middle dose group mice increased SOD activity（ p 〈0.05）, low dose group mice CAT activity returning to normal in heart, kidney lplasma, middle dose group mice GSH-Px activity significantly increased in liver, spleen, plasma （p 〈 0.01）, different doses group mice MDA content effectively reduce in tissue（p 〈 0.05）, middle dose group mice LDH activity returning to normal in tissue. But overall effect was not in competition with leucogen tablets. In conclusion,AKP could greatly protect the body, reduce the adverse effects of oxidative damage on organism, and to provide a scientific basis for further development and utilization.