补肾活血健骨方含药血清对人成骨细胞增殖、分化及矿化功能的影响

Effect of Bushen Huoxue Jiangu Decoction Drug Serum on Osteoblast Proliferation,Differentiation,Maturation and Mineralization in Vitro

目的观察补肾活血健骨方含药血清对体外培养的人成骨细胞增殖、分化和矿化功能的影响,为其治疗骨质疏松症提供理论依据。方法取骨质疏松症合并股骨颈骨折患者松质骨进行成骨细胞培养,构建成骨细胞分化模型;制备低、中、高3种浓度的补肾活血健骨方含药血清;采用MTT法、细胞上清液ALP测定和茜素红染色观察含药血清对成骨细胞的影响。结果加入含药血清干预细胞生长48h后,MTT检测显示中、高剂量组出现不同程度的细胞增殖现象,并存在一定程度的剂量依赖;作用72h后,低、中、高3个剂量组ALP活性增高,高剂量组茜素红染色钙盐沉积明显增多。结论补肾活血健骨方含药血清具有促进体外培养的人成骨细胞增殖、分化及矿化的作用。

OBJECTIVE To observe the effects of Bushen Huoxue Jiangu Decoction drug serum on osteoblast proliferation,differentiation,maturation and mineralization in vitro,which will provide the theoretical basis for osteoporosis treatment.METHODS Cancellous bone specimens from the femoral head were obtained from patients with femoral neck fracture merger osteoporosis,and the osteoblast cells were seeded and cultured in vitro to establish the osteoblast differentiation model.High,middle and low doses of Bushen Huoxue Jiangu Decoction drug serum were prepared.MTT method,ALP activity assay and alizarin red staining were used to observe the proliferation,activity of ALP and the number of mineral node of cultured osteoblasts in vitro.RESULTS MTT assay displayed different degrees of osteoblast proliferation in high and middle dose groups and showed some dose dependence after 48 hof drug intervention.The activities of ALP in high,middle and low dose groups obviously increased after 72 hof drug intervention.The deposition of calcium salt crystal in alizarin red staining obviously increased in high dose group after 72 hof drug intervention.CONCLUSION Bushen Huoxue Jiangu Decoction drug serum can promote osteoblast proliferation,differentiation,maturation and mineralization in vitro.