复层牙周膜细胞膜片组织再生的实验性研究

An Experimental Study of Multilayer Ligamental Periodontal Cell Sheets on Tissue Regeneration

目的:制备复层牙周膜干细胞膜片(MUCPs)与单层牙周膜干细胞膜片(MCPs),并评价其生物学活性和组织再生能力。方法:将构建的2种细胞膜片通过免疫组化和扫描电镜等方法观察其生物学活性。再将2种膜片分别与2种支架材料陶瓷牛骨(CBB)与预处理牙本质片(TDM)复合构建不同的生物性牙根,植入裸鼠皮下,观察组织再生情况。结果:体外结果显示MUCPs高表达Col-I、COL-III、Fibronectin、Laminin等细胞外基质的结构蛋白。体内结果显示在复层细胞膜片组可以观察到再生出更明显的类似于牙周复合组织的矿化沉积和牙周膜样纤维。结论:MUCPs具有更为良好的生物学活性和组织再生能力,将为临床牙周组织缺损的治疗提供一种新的治疗方法。

Objective：To fabricate multilayer periodontal ligament stem cell pellets（MUCPs）and monolayer periodontal ligament stem cell pellets（MCPs）,and to evaluate their biological activity and regeneration potential.Methods：Biological properties of two kinds of cell sheets were observed by immunohistochemistry and scanning electron microscopy.And then the cell sheets were combined with CBB to construct biological roots.Tissue regeneration in the nude mice after ectopic transplantation was observed.Results：The in vitro experiments showed higher levels of structural proteins in the extracellular matrix composed of COL-I,COL-III,fibronectin and laminin in the MUCPs.Upon transplantation,formation of mineralized nodules and periodontal fibrous tissue similar to the periodontal tissues was more obvious in the MUCPs than that in MCPs in vivo.Conclusion：MUCPs presented a good biological activity and it may be a promising alternative in periodontal repair for future clinical application.