柳州市16例吸毒者HIV-1病毒流行株亚型分布及耐药情况分析

Analysis of HIV-1 Virus Epidemic Strain Subtype Distribution in 16 Cases of Drug Users in Liuzhou and Drug Resistance

目的：了解2011-2013年柳州市吸毒人群人类免疫缺陷病毒1（human immunodeficiency virus 1, HIV-1）亚型分布和毒株耐药情况。方法从2011-2013年柳州市吸毒人群HIV-1感染者中，随机抽取16例作为研究对象，从血浆中抽提核酸RNA，用RT-PCR进行gag及pol基因扩增，并对扩增产物进行测序和序列分析。结果16例标本中存在2种HIV-1亚型：重组亚型CRF01_AE 14例（87.5%），重组亚型CRF07_BC 2例（12.5%）。6株发生相关耐药突变，主要发生在NRTIs和NNRTIs相关突变。结论2011~2013年柳州市吸毒人群HIV-1感染主要以CRF01_AE为主，主要在NRTIs和NNRTIs相关耐药突变。

Objective To know the HIV-1 virus epidemic strain subtype distribution in drug users in Liuzhou from 2011 to 2013 and drug resistance. Methods 16 cases of HIV-1 infected persons in the drug users in Liuzhou from 2011 to 2013 were randomly selected as the research objects, and the nucleic acid RNA was extracted from blood plasma, RT-PCR was used for gag and pol gene amplification, and the amplification products were given sequencing and sequential analysis. Re-sults In the 16 cases of specimens, there were 2 HIV-1 subtypes, 14 cases of reconstruction subtypes CRF01_AE （87.5%）, and 2 cases of reconstruction subtypes CRF07_BC（12.5%）, the related drug resistance mutation occurred to 6 strains, and NRTIs and NNRTIs related mutations were the main. Conclusion The HIV-1 infection of drug users in Liuzhou from 2011 to 2013 was mostly CRF01_AE, and the NRTIs and NNRTIs related mutations were the main.