摘要
利用溶液浇铸-粒子滤沥法制备不同ATP含量(质量分数为0%、10%、30%)的凹凸棒石/Ⅰ型胶原/聚己内酯(ATP/ColⅠ/PCL)复合材料。通过SEM、FT-IR、孔隙率、吸水膨胀率、三点弯曲和压缩实验对材料进行结构表征和性能评价。利用细胞和动物皮下埋植实验评价生物相容性。结果显示:支架材料为多孔结构,复合ATP后,材料表面粗糙。30%ATP材料的孔隙率、吸水膨胀率与0%、10%ATP相比显著降低,而最大弯曲应力和压缩应力显著增强(P<0.05)。SEM结果显示30%ATP材料表面细胞呈梭形黏附生长;30%ATP材料植入SD大鼠皮下21d后,材料孔隙中有大量非炎性细胞侵润且形成组织,与0%、10%ATP材料相比,材料仅有极少部分降解。凹凸棒石/Ⅰ型胶原/聚己内酯复合支架材料具有多孔性、较强的力学性能、良好的理化性能和生物相容性,有望成为一种理想的骨组织工程支架材料。
The attapulgite/type I collagen/poly（caprolactone） composite scaffolds with different ATP contents （mass fraction of 0 %,10 %, 30%） were prepared by the solution perfusion-salt-Ieaching process. The characterizations of materials were conducted by SEM, FT-IR, water absorption expansion rate, porosity, three-point bending and compression test. The biocompatibility was evaluated cell and animal experiments. The results showed that the surface of the materials was porous, and rough after composite ATP. The porosity, water absorption were significantly de- creased compared with 0%, 10% ATP（P〈0. 05）, while maximal compressive and bending stress were increased. SEM results showed that 30% ATP material had suitable cell adhesion property. The animal experiments showed that materials abounded in the inflammatory cells invasion and pore forming organization, and there was tiny part of the degradation in 30% ATP. ATP/Col I/PCL composite has porous structure, good physical, chemical properties, strong mechanical properties and biocompatibility, and may be used as an ideal scaffold for bone tissue engineering.
基金
甘肃省科技重大专项项目(1203FKDA036)
关键词
凹凸棒石
I型胶原
聚己内酯
复合支架材料
生物相容性
attapulgite
type I collagen
poly（caprolactone）
composite scaffold material
biocompatibility
