创伤性失血性休克对机体抗氧化酶系统影响的实验研究

Experimental Study on the Influences of Antioxidant Enzyme Systems of Organism on Traumatic Hemorrhagic shock

目的探讨创伤性失血性休克(traumatic hemorrhagic shock,THS)对机体抗氧化酶系统的影响。方法选择普通级新西兰兔11只,通过急性失血方式建立收缩压下降曲线,形成THS动物模型,测定收缩压基础值(T1),当收缩压降至基础值2/3(T2)、基础值1/2(T3)、基础值1/3(T4)时采集血样,采用酶联免疫吸附分析检测各位置血清超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(Catalase,CAT)、谷胱甘肽过氧化物酶(glutathione oxidation r e d u c t a s e,G S H-P x)水平,不同位置检测数据以A、B、C、D分组,进行统计分析。结果 T-S O D:A组低于B组、B组高于C组、C组高于D组,各时间水平间的差异无统计学意义(P>0.05);CAT:A组高于B组、B组高于C组、C组高于D组,T 1-T 2、T 2-T 3、T 3-T 4各组间的差异无统计学意义(P>0.05),其余组间的差异有统计学意义(P<0.05);G S HP x:A组高于B组、B组高于C组、C组高于D组,T 1、T 2间差异无统计学意义(P>0.05),其余组间差异有统计学意义(P<0.05)。结论选择收缩压下降曲线建立THS模型,THS发生过程对机体抗氧化酶系统具有显著影响,可导致CAT、G S H-P x活性显著下降及S O D活性下降。T H S发生过程中失血量与C A T、G S H-P x活性间存在相关性。

Objective To study the influence of traumatic hemorrhagic shock（THS）on antioxidant enzymes. Methods The model of the THS with gradient decrease of the systolic blood pressure was etablished in11 Newzealand rabbits.The arterial blood pressures in the different time following THS were measured with the systolic pressures of2/3 （T2）,1/2（T3）,and1/3（T4）of the basic systolic blood pressure Superoxide dismutase（SOD）,hydrogen peroxide enzyme（CAT）,and glutathione oxidation reductase（GSH-Px）levels in the plasma were determined by the double antibody sandwich method of enzyme-labeled immunosorbent assay（ELISH）.Results T-SOD：The levels of the antioxidant enzymes were lower in Group A than in group B; group B was higher than group C; and group C was higher than group D. There was no significant difference between the time level（P〉0.05）.In CAT detection,it was higher in Group A than in group B,group B was higher than group C,group C was higher than group D,there was no significant difference between the T1-T2,T2-T3,and T3-T4（P〉0.05）. The significant difference,however,was noted between the other groups（P 〈0.05）. In GSH-Px tests,Group A was found to be higher than group B,group B was higher than group C, group C was higher than group D,there was no significant difference between the T1 and T2（P〉0.05）,and A significant difference was seen between the other groups（P〈0.05）.Conclusion When the systolic blood pressure decline curve was used for THS and antioxidant detection in rabbits,THS has a significant affect on the antioxidant enzyme systemshowing a significant decrease of CAT,GSH-Px and SOD,and activity decreased. Blood loss coincides with decrease of CAT and GSH-Px on THS.