桑白皮多糖对呼吸道合胞病毒肺炎小鼠肺组织病理和外周血T细胞亚群的影响

Effects of Morus alba L. polysaccharide on lung tissue and T lymphocyte subtype in mice infected by respiratory syncytial virus

目的通过研究桑白皮多糖对呼吸道合胞病毒(RSV)肺炎BALB/c小鼠肺组织病理形态和外周血T细胞亚群的影响,探讨桑白皮多糖对BALB/c小鼠RSV肺炎的干预机制。方法 BALB/c小鼠采用随机数字表法分为正常组、模型组、高剂量组、中剂量组、低剂量组,每组10只;除正常组外,用RSV(long株)经鼻腔接种,建立小鼠RSV肺炎模型。于感染当天,高、中、低剂量组分别给予桑白皮多糖182、91、45.5 mg·kg-1·d-1灌胃,正常组与模型组给予等体积生理盐水灌胃,每天1次,于感染第7天取外周血进行T细胞亚群的检测及肺组织进行HE染色。结果桑白皮多糖能有效地减少肺泡壁炎细胞的浸润,改善肺组织的炎症状况。高、中、低剂量组与模型组比较,高剂量组CD3+T细胞百分率为(50.35±8.97)%、CD4+T/CD8+T为(3.52±0.79),差异有统计学意义(P<0.05);中剂量组CD3+T细胞百分率为(55.05±5.94)%、CD4+T细胞百分率为(20.39±5.56)%、CD4+T/CD8+T为(2.88±0.56),差异有统计学意义(P<0.05);低剂量组只有CD4+T/CD8+T为(3.49±0.45),差异有统计学意义(P<0.05)。结论桑白皮多糖可以改善肺炎小鼠肺组织病理变化,能够调节机体细胞免疫。

Objective To investigate the effects of Morus alba L. polysaccharide on lung tissue and T lymphocyte subtype in BALB / c mice infected by respiratory syncytial virus. Methods Fifty young BALB / c mice were randomized into normal group,model group,high dosage group,middle dosage group and low dosage group. Except for the normal group,RSV was intranasally inoculated to mice. Aqueous solution of high,medium and low doses of Morus alba L. polysaccharide（ 182,91,45. 5 mg · kg- 1·d- 1） were applied for the high,middle,low dosage group by gavage,and 0. 9% sodium chloride solution for the normal group and model group by gavage,for a total of seven days. And then,pathological change and T lymphocyte subtype were detected. Results Morus alba L. polysaccharide could reduce the infiltration of inflammatory cells in the alveolar wall. Compared with the model group,the percentage of CD3 ＋ T（ 50. 35 ±8. 97） %,CD4 ＋ T / CD8 ＋ T（ 3. 52 ± 0. 79） in high dosage group were significantly different（ P〈0. 05）,the percentage of CD3 ＋ T（ 55. 05 ± 5. 94） %,CD4 ＋ T（ 20. 39 ± 5. 56） %,CD4 ＋ T/CD8 ＋ T（ 2. 88 ± 0. 56） in middle dosage group were significantly different（ P〈0. 05）,and CD4 ＋ T/CD8 ＋ T（ 3. 49 ± 0. 45） in low dosage group were significantly different（ P〈0. 05）. Conclusion Morus alba L. polysaccharide can improve the pathological change of lung tissue and promote cell immune adjustment in the infected mice.